Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells’ ozone response

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Nuhkat , M , Brosché , M , Stoelzle-Feix , S , Dietrich , P , Hedrich , R , Roelfsema , M R G & Kollist , H 2021 , ' Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells’ ozone response ' , New Phytologist , vol. 232 , no. 4 , pp. 1692-1702 . https://doi.org/10.1111/nph.17711

Title: Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells’ ozone response
Author: Nuhkat, Maris; Brosché, Mikael; Stoelzle-Feix, Sonja; Dietrich, Petra; Hedrich, Rainer; Roelfsema, M.Rob G.; Kollist, Hannes
Contributor: University of Helsinki, Viikki Plant Science Centre (ViPS)
University of Helsinki, University of Tartu
Date: 2021-11
Language: eng
Number of pages: 11
Belongs to series: New Phytologist
ISSN: 0028-646X
URI: http://hdl.handle.net/10138/335823
Abstract: Plant stress signalling involves bursts of reactive oxygen species (ROS), which can be mimicked by the application of acute pulses of ozone. Such ozone-pulses inhibit photosynthesis and trigger stomatal closure in a few minutes, but the signalling that underlies these responses remains largely unknown. We measured changes in Arabidopsis thaliana gas exchange after treatment with acute pulses of ozone and set up a system for simultaneous measurement of membrane potential and cytosolic calcium with the fluorescent reporter R-GECO1. We show that within 1 min, prior to stomatal closure, O3 triggered a drop in whole-plant CO2 uptake. Within this early phase, O3 pulses (200–1000 ppb) elicited simultaneous membrane depolarization and cytosolic calcium increase, whereas these pulses had no long-term effect on either stomatal conductance or photosynthesis. In contrast, pulses of 5000 ppb O3 induced cell death, systemic Ca2+ signals and an irreversible drop in stomatal conductance and photosynthetic capacity. We conclude that mesophyll cells respond to ozone in a few seconds by distinct pattern of plasma membrane depolarizations accompanied by an increase in the cytosolic calcium ion (Ca2+) level. These responses became systemic only at very high ozone concentrations. Thus, plants have rapid mechanism to sense and discriminate the strength of ozone signals. © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation
Subject: 11831 Plant biology
Arabidopsis thaliana
Ca2+ indicator
Ca2+ signalling
membrane depolarization
mesophyll
ozone
reactive oxygen species (ROS)
NADPH OXIDASE
ANION CHANNEL
ARABIDOPSIS-THALIANA
INDUCED TRANSIENT
PLASMA-MEMBRANE
PERMEABLE CHANNELS
HYDROGEN-PEROXIDE
GUARD-CELLS
ABSCISIC-ACID
K+ CHANNELS
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