Mechano-chemical feedback mediated competition for BMP signalling leads to pattern formation

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http://hdl.handle.net/10138/335907

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Toddie-Moore , D J , Montanari , M P , Tran , N V , Brik , E M , Antson , H , Salazar-Ciudad , I & Shimmi , O 2022 , ' Mechano-chemical feedback mediated competition for BMP signalling leads to pattern formation ' , Developmental Biology , vol. 481 , pp. 43-51 . https://doi.org/10.1016/j.ydbio.2021.09.006

Title: Mechano-chemical feedback mediated competition for BMP signalling leads to pattern formation
Author: Toddie-Moore, Daniel J.; Montanari, Martti P.; Tran, Ngan Vi; Brik, Evgeniy M.; Antson, Hanna; Salazar-Ciudad, Isaac; Shimmi, Osamu
Other contributor: University of Helsinki, Morphogenesis and cellular dynamics
University of Helsinki, Institute of Biotechnology
University of Helsinki, Univ Tartu, University of Tartu, Estonian Genome Ctr, Dept Biotechnol, Inst Genom,Inst Mol & Cell Biol
University of Helsinki, Biosciences
University of Helsinki, Institute of Biotechnology


Date: 2022-01
Language: eng
Number of pages: 9
Belongs to series: Developmental Biology
ISSN: 0012-1606
DOI: https://doi.org/10.1016/j.ydbio.2021.09.006
URI: http://hdl.handle.net/10138/335907
Abstract: Developmental patterning is thought to be regulated by conserved signalling pathways. Initial patterns are often broad before refining to only those cells that commit to a particular fate. However, the mechanisms by which pattern refinement takes place remain to be addressed. Using the posterior crossvein (PCV) of the Drosophila pupal wing as a model, into which bone morphogenetic protein (BMP) ligand is extracellularly transported to instruct vein patterning, we investigate how pattern refinement is regulated. We found that BMP signalling induces apical enrichment of Myosin II in developing crossvein cells to regulate apical constriction. Live imaging of cellular behaviour indicates that changes in cell shape are dynamic and transient, only being maintained in those cells that retain vein fate competence after refinement. Disrupting cell shape changes throughout the PCV inhibits pattern refinement. In contrast, disrupting cell shape in only a subset of vein cells can result in a loss of BMP signalling. We propose that mechano-chemical feedback leads to competition for the developmental signal which plays a critical role in pattern refinement.
Subject: Drosophila melanogaster
epithelial cells
apical constriction
BMP signalling
Cell competition
TWISTED GASTRULATION
MORPHOGEN
COORDINATION
CONTRACTION
MECHANISM
APOPTOSIS
TRANSPORT
TENSION
FAMILY
GROWTH
1184 Genetics, developmental biology, physiology
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