From Thermogelling Hydrogels toward Functional Bioinks : Controlled Modification and Cytocompatible Crosslinking

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Hahn , L , Beudert , M , Gutmann , M , Kessler , L , Stahlhut , P , Fischer , L , Karakaya , E , Lorson , T , Thievessen , I , Detsch , R , Luehmann , T & Luxenhofer , R 2021 , ' From Thermogelling Hydrogels toward Functional Bioinks : Controlled Modification and Cytocompatible Crosslinking ' , Macromolecular Bioscience , vol. 21 , no. 10 , 2100122 . https://doi.org/10.1002/mabi.202100122

Title: From Thermogelling Hydrogels toward Functional Bioinks : Controlled Modification and Cytocompatible Crosslinking
Author: Hahn, Lukas; Beudert, Matthias; Gutmann, Marcus; Kessler, Larissa; Stahlhut, Philipp; Fischer, Lena; Karakaya, Emine; Lorson, Thomas; Thievessen, Ingo; Detsch, Rainer; Luehmann, Tessa; Luxenhofer, Robert
Contributor: University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
Date: 2021-10
Language: eng
Number of pages: 9
Belongs to series: Macromolecular Bioscience
ISSN: 1616-5187
URI: http://hdl.handle.net/10138/335937
Abstract: Hydrogels are key components in bioink formulations to ensure printability and stability in biofabrication. In this study, a well-known Diels-Alder two-step post-polymerization modification approach is introduced into thermogelling diblock copolymers, comprising poly(2-methyl-2-oxazoline) and thermoresponsive poly(2-n-propyl-2-oxazine). The diblock copolymers are partially hydrolyzed and subsequently modified by acid/amine coupling with furan and maleimide moieties. While the thermogelling and shear-thinning properties allow excellent printability, trigger-less cell-friendly Diels-Alder click-chemistry yields long-term shape-fidelity. The introduced platform enables easy incorporation of cell-binding moieties (RGD-peptide) for cellular interaction. The hydrogel is functionalized with RGD-peptides using thiol-maleimide chemistry and cell proliferation as well as morphology of fibroblasts seeded on top of the hydrogels confirm the cell adhesion facilitated by the peptides. Finally, bioink formulations are tested for biocompatibility by incorporating fibroblasts homogenously inside the polymer solution pre-printing. After the printing and crosslinking process good cytocompatibility is confirmed. The established bioink system combines a two-step approach by physical precursor gelation followed by an additional chemical stabilization, offering a broad versatility for further biomechanical adaptation or bioresponsive peptide modification.
Subject: 116 Chemical sciences
biofabrication
bioprinting
chemical crosslinking
hydrogels
NONIONIC HYDROGEL
3D
POLYOXAZOLINE
2-METHYL-2-OXAZOLINE
POLY(2-OXAZOLINE)S
CYTOTOXICITY
BIOMATERIALS
STRATEGIES
DELIVERY
GELATION
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