Sarcoplasmic and myofibril-bound calpains during storage of pork longissimus muscle : New insights on protein degradation

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Lyu , J & Ertbjerg , P 2022 , ' Sarcoplasmic and myofibril-bound calpains during storage of pork longissimus muscle : New insights on protein degradation ' , Food Chemistry , vol. 372 , 131347 . https://doi.org/10.1016/j.foodchem.2021.131347

Title: Sarcoplasmic and myofibril-bound calpains during storage of pork longissimus muscle : New insights on protein degradation
Author: Lyu, Jian; Ertbjerg, Per
Other contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition

Date: 2022-03-15
Language: eng
Number of pages: 6
Belongs to series: Food Chemistry
ISSN: 0308-8146
DOI: https://doi.org/10.1016/j.foodchem.2021.131347
URI: http://hdl.handle.net/10138/335958
Abstract: The subcellular distribution of calpain-1 and -2 and the proteolytical activity of myofibril-bound calpains in pork were investigated during 12 days cold storage. The content of sarcoplasmic calpain-1 decreased during storage while myofibril-bound calpain-1 content first increased (P < 0.05) to 17% of that of 12 h-sarcoplasmic calpain-1 on day 6 followed by a gradual decrease with subsequent storage, suggesting that calpain-1 gradually translocated from sarcoplasm to myofibrils during the initial 6 days of postmortem storage. Intact desmin decreased (P < 0.05) after incubation of myofibrils with 0.05 mM Ca2+, and this was more pronounced with 5 mM Ca2+ (P < 0.05). Ca2+ titration curves of day 6 myofibrils showed two distinct proteolytic activities becoming activated in the range 0.03 to 0.06 mM and 0.4 to 0.8 mM Ca2+, respectively. The results suggest that both calpain-1 and calpain-2 binds to myofibrils during storage and subsequently degrade structural proteins including desmin.
Subject: 416 Food Science
Calcium
Calpain-1
Calpain-2
Desmin
Myofibril-bound calpain
Postmortem proteolysis
POSTMORTEM STORAGE
WATER-HOLDING CAPACITY
TENDERIZATION
PROTEOLYSIS
MEAT
TEMPERATURE
MU-CALPAIN
CALLIPYGE
CALPASTATIN
TENDERNESS
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