Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum

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Hafeez , M N , Khan , M A , Sarwar , B , Hassan , S , Ali , Q , Husnain , T & Rashid , B 2021 , ' Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum ' , Scientific Reports , vol. 11 , no. 1 , 20466 . https://doi.org/10.1038/s41598-021-99900-x

Title: Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum
Author: Hafeez, Muhammad Nadeem; Khan, Mohsin Ahmad; Sarwar, Bilal; Hassan, Sameera; Ali, Qurban; Husnain, Tayyab; Rashid, Bushra
Contributor organization: Drug Research Program
Division of Pharmaceutical Chemistry and Technology
Faculty of Pharmacy
Date: 2021-10-14
Language: eng
Number of pages: 19
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-021-99900-x
URI: http://hdl.handle.net/10138/336378
Abstract: Gossypium arboreum is considered a rich source of stress-responsive genes and the EST database revealed that most of its genes are uncharacterized. The full-length Gossypium universal stress protein-2 (GUSP-2) gene (510 bp) was cloned in E. coli and Gossypium hirsutum, characterized and point mutated at three positions, 352-354, Lysine to proline (M1-usp-2) & 214-216, aspartic acid to serine (M2-usp-2) & 145-147, Lysine to Threonine (M3-usp-2) to study its role in abiotic stress tolerance. It was found that heterologous expression of one mutant (M1-usp-2) provided enhanced tolerance against salt and osmotic stresses, recombinant cells have higher growth up to 10-5dilution in spot assay as compared to cells expressing W-usp-2 (wild type GUSP-2), M2-usp-2 and M3-usp-2 genes. M1-usp-2 gene transcript profiling exhibited significant expression (8.7 fold) in CIM-496-Gossypium hirsutum transgenic plants and enhance drought tolerance. However, little tolerance against heat and cold stresses in bacterial cells was observed. The results from our study concluded that the activity of GUSP-2 was enhanced in M1-usp-2 but wipe out in M2-usp-2 and M3-usp-2 response remained almost parallel to W-usp-2. Further, it was predicted through in silico analysis that M1-usp-2, W-usp-2 and M3-usp-2 may be directly involved in stress tolerance or function as a signaling molecule to activate the stress adaptive mechanism. However, further investigation will be required to ascertain its role in the adaptive mechanism of stress tolerance.
Subject: WATER-DEFICIT STRESS
MEMBRANE THERMOSTABILITY
HETEROLOGOUS EXPRESSION
SALICORNIA-BRACHIATA
GOSSYPIUM-ARBOREUM
EXTREME HALOPHYTE
ESCHERICHIA-COLI
HIGH-TEMPERATURE
HEAT TOLERANCE
PROTEIN
1182 Biochemistry, cell and molecular biology
317 Pharmacy
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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