Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide)

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http://hdl.handle.net/10138/336511

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Semenyuk , P I , Kurochkina , L P , Mäkinen , L , Muronetz , V I & Hietala , S 2021 , ' Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide) ' , Polymers , vol. 13 , no. 20 , 3601 . https://doi.org/10.3390/polym13203601

Title: Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide)
Author: Semenyuk, Pavel I.; Kurochkina, Lidia P.; Mäkinen, Lauri; Muronetz, Vladimir I.; Hietala, Sami
Other contributor: University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry

Date: 2021-10
Language: eng
Number of pages: 10
Belongs to series: Polymers
ISSN: 2073-4360
DOI: https://doi.org/10.3390/polym13203601
URI: http://hdl.handle.net/10138/336511
Abstract: A prospective technology for reversible enzyme complexation accompanied with its inactivation and protection followed by reactivation after a fast thermocontrolled release has been demonstrated. A thermoresponsive polymer with upper critical solution temperature, poly(N-acryloyl glycinamide) (PNAGA), which is soluble in water at elevated temperatures but phase separates at low temperatures, has been shown to bind lysozyme, chosen as a model enzyme, at a low temperature (10 & DEG;C and lower) but not at room temperature (around 25 & DEG;C). The cooling of the mixture of PNAGA and lysozyme solutions from room temperature resulted in the capturing of the protein and the formation of stable complexes; heating it back up was accompanied by dissolving the complexes and the release of the bound lysozyme. Captured by the polymer, lysozyme was inactive, but a temperature-mediated release from the complexes was accompanied by its reactivation. Complexation also partially protected lysozyme from proteolytic degradation by proteinase K, which is useful for biotechnological applications. The obtained results are relevant for important medicinal tasks associated with drug delivery such as the delivery and controlled release of enzyme-based drugs.
Subject: thermosensitive polymers
enzyme complexation
controlled release
reversible inactivation
UCST polymers
stimuli-responsive polymers
CRITICAL SOLUTION TEMPERATURE
PHASE-TRANSITION
POLYMERS
IMMOBILIZATION
POLYMERIZATION
PARTICLES
MICROGELS
HYDROGEL
CARRIERS
RELEASE
116 Chemical sciences
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