Comparative Analysis of Vascular Mimicry in Head and Neck Squamous Cell Carcinoma: In Vitro and In Vivo Approaches

Show full item record



Permalink

http://hdl.handle.net/10138/336747

Citation

Hujanen , R , Almahmoudi , R , Salo , T & Salem , A 2021 , ' Comparative Analysis of Vascular Mimicry in Head and Neck Squamous Cell Carcinoma: In Vitro and In Vivo Approaches ' , Cancers , vol. 13 , no. 9 , 4747 . https://doi.org/10.3390/cancers13194747

Title: Comparative Analysis of Vascular Mimicry in Head and Neck Squamous Cell Carcinoma: In Vitro and In Vivo Approaches
Author: Hujanen, Roosa; Almahmoudi, Rabeia; Salo, Tuula; Salem, Abdelhakim
Contributor: University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, HUSLAB
University of Helsinki, HUSLAB
University of Helsinki, Clinicum
Date: 2021-09-23
Language: eng
Belongs to series: Cancers
ISSN: 2072-6694
URI: http://hdl.handle.net/10138/336747
Abstract: Tissue vasculature provides the main conduit for metastasis in solid tumours including head and neck squamous cell carcinoma (HNSCC). Vascular mimicry (VM) is an endothelial cell (EC)-independent neovascularization pattern, whereby tumour cells generate a perfusable vessel-like meshwork. Yet, despite its promising clinical utility, there are limited approaches to better identify VM in HNSCC and what factors may influence such a phenomenon in vitro. Therefore, we employed different staining procedures to assess their utility in identifying VM in tumour sections, wherein mosaic vessels may also be adopted to further assess the VM-competent cell phenotype. Using 13 primary and metastatic HNSCC cell lines in addition to murine- and human-derived matrices, we elucidated the impact of the extracellular matrix, tumour cell type, and density on the formation and morphology of cell-derived tubulogenesis in HNSCC. We then delineated the optimal cell numbers needed to obtain a VM meshwork in vitro, which revealed cell-specific variations and yet consistent expression of the EC marker CD31. Finally, we proposed the zebrafish larvae as a simple and cost-effective model to evaluate VM development in vivo. Taken together, our findings offer a valuable resource for designing future studies that may facilitate the therapeutic exploitation of VM in HNSCC and other tumours.
Subject: 3122 Cancers
head and neck squamous cell carcinoma
VASCULAR MIMICRY
MATRIGEL
Myogel
ZEBRAFISH
METASTASIS
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Hujanen_et_al._2021.pdf 5.539Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record