An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut

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http://hdl.handle.net/10138/336985

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Viitanen , A I , Gullmets , J , Morikka , J G A , Katajisto , P , Mattila , J & Hietakangas , V 2021 , ' An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut ' , Cell Reports Methods , vol. 1 , no. 5 . https://doi.org/10.1016/j.crmeth.2021.100059

Title: An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut
Author: Viitanen, Arto Ilmari; Gullmets, Josef; Morikka, Jack George Anthony; Katajisto, Pekka; Mattila, Jaakko; Hietakangas, Ville
Contributor organization: Molecular and Integrative Biosciences Research Programme
Nutrient sensing laboratory
Centre of Excellence in Stem Cell Metabolism
Helsinki Institute of Life Science HiLIFE
Date: 2021-09-27
Language: eng
Belongs to series: Cell Reports Methods
ISSN: 2667-2375
DOI: https://doi.org/10.1016/j.crmeth.2021.100059
URI: http://hdl.handle.net/10138/336985
Abstract: The intestine is divided into functionally distinct regions along the anteroposterior (A/P) axis. How the regional identity influences the function of intestinal stem cells (ISCs) and their offspring remain largely unresolved. We introduce an imaging-based method, “Linear Analysis of Midgut” (LAM), which allows quantitative, regionally defined cellular phenotyping of the whole Drosophila midgut. LAM transforms image-derived cellular data from three-dimensional midguts into a linearized representation, binning it into segments along the A/P axis. Through automated multivariate determination of regional borders, LAM allows mapping and comparison of cellular features and frequencies with subregional resolution. Through the use of LAM, we quantify the distributions of ISCs, enteroblasts, and enteroendocrine cells in a steady-state midgut, and reveal unprecedented regional heterogeneity in the ISC response to a Drosophila model of colitis. Altogether, LAM is a powerful tool for organ-wide quantitative analysis of the regional heterogeneity of midgut cells.
Subject: 1184 Genetics, developmental biology, physiology
Midgut
Organ-wide
imaging
stem cell
injury response
drosophila
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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