Phage Treatment Trial to Eradicate LA-MRSA from Healthy Carrier Pigs

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Tuomala , H , Verkola , M , Meller , A , Van der Auwera , J , Patpatia , S , Järvinen , A , Skurnik , M , Heikinheimo , A & Kiljunen , S 2021 , ' Phage Treatment Trial to Eradicate LA-MRSA from Healthy Carrier Pigs ' , Viruses (Basel) , vol. 13 , no. 10 , 1888 . https://doi.org/10.3390/v13101888

Title: Phage Treatment Trial to Eradicate LA-MRSA from Healthy Carrier Pigs
Author: Tuomala, Henni; Verkola, Marie; Meller, Anna; Van der Auwera, Jasper; Patpatia, Sheetal; Järvinen, Asko; Skurnik, Mikael; Heikinheimo, Annamari; Kiljunen, Saija
Contributor organization: HUSLAB
Department of Bacteriology and Immunology
HUMI - Human Microbiome Research
Food Hygiene and Environmental Health
Helsinki One Health (HOH)
Helsinki Institute of Life Science HiLIFE, Infra
Research Programs Unit
HUS Inflammation Center
Department of Medicine
Infektiosairauksien yksikkö
Mikael Skurnik / Principal Investigator
Medicum
Zoonotic Antimicrobial Resistance
Date: 2021-10
Language: eng
Number of pages: 15
Belongs to series: Viruses (Basel)
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v13101888
URI: http://hdl.handle.net/10138/337333
Abstract: The increase of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) causes a threat to human health. LA-MRSA can be transmitted from animals to animal caretakers, which may further spread MRSA to communities and health care facilities. The objective of this work was to study the efficacy of phage treatment in the eradication of LA-MRSA from healthy carrier pigs. A total of 19 MRSA -positive weanling pigs were assigned to a test (n = 10) and a control group (n = 9). A phage cocktail containing three Staphylococcus phages, or a control buffer was administered to the nares and skin of the pigs three times every two days, after which the phage and MRSA levels in nasal and skin swab samples were monitored for a three-week period. The sensitivity of the strains isolated during the follow-up period to the phage cocktail and each phage individually was analyzed and the pig sera were tested for antibodies against the phages used in the cocktail. The phage treatment did not cause any side effects to the pigs. Phages were found in the skin and nasal samples on the days following the phage applications, but there was no reduction in the MRSA levels in the sampled animals. Phage-resistant strains or phage-specific antibodies were not detected during the experiment. The MRSA load in these healthy carrier animals was only 10–100 CFU/swab or nasal sample, which was likely below the replication threshold of phages. The effectiveness of phage treatment to eradicate MRSA from the pigs could thus not be (reliably) determined.
Subject: 413 Veterinary science
11832 Microbiology and virology
LA-MRSA
phage
phage therapy
pig
antibiotic resistance
RESISTANT STAPHYLOCOCCUS-AUREUS
YERSINIA-ENTEROCOLITICA
BACTERIOPHAGE THERAPY
HIGH-RISK
VFDB
VETERINARIANS
INFECTIONS
EFFICACY
MODEL
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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