High-resolution targeted bisulfite sequencing reveals blood cell type-specific DNA methylation patterns in IL13 and ORMDL3

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http://hdl.handle.net/10138/337346

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Soderhall , C , Reinius , LE , Salmenpera , P , Gentile , M , Acevedo , N , Konradsen , JR , Nordlund , B , Hedlin , G , Scheynius , A , Myllykangas , S & Kere , J 2021 , ' High-resolution targeted bisulfite sequencing reveals blood cell type-specific DNA methylation patterns in IL13 and ORMDL3 ' , Clinical epigenetics , vol. 13 , no. 1 , 106 . https://doi.org/10.1186/s13148-021-01093-7

Title: High-resolution targeted bisulfite sequencing reveals blood cell type-specific DNA methylation patterns in IL13 and ORMDL3
Author: Soderhall, C; Reinius, LE; Salmenpera, P; Gentile, M; Acevedo, N; Konradsen, JR; Nordlund, B; Hedlin, G; Scheynius, A; Myllykangas, S; Kere, J
Contributor organization: STEMM - Stem Cells and Metabolism Research Program
Juha Kere / Principal Investigator
Research Programs Unit
Date: 2021-05-10
Language: eng
Number of pages: 10
Belongs to series: Clinical epigenetics
ISSN: 1868-7075
DOI: https://doi.org/10.1186/s13148-021-01093-7
URI: http://hdl.handle.net/10138/337346
Abstract: Background Methylation of DNA at CpG sites is an epigenetic modification and a potential modifier of disease risk, possibly mediating environmental effects. Currently, DNA methylation is commonly assessed using specific microarrays that sample methylation at a few % of all methylated sites. Methods To understand if significant information on methylation can be added by a more comprehensive analysis of methylation, we set up a quantitative method, bisulfite oligonucleotide-selective sequencing (Bs-OS-seq), and compared the data with microarray-derived methylation data. We assessed methylation at two asthma-associated genes, IL13 and ORMDL3, in blood samples collected from children with and without asthma and fractionated white blood cell types from healthy adult controls. Results Our results show that Bs-OS-seq can uncover vast amounts of methylation variation not detected by commonly used array methods. We found that high-density methylation information from even one gene can delineate the main white blood cell lineages. Conclusions We conclude that high-resolution methylation studies can yield clinically important information at selected specific loci missed by array-based methods, with potential implications for future studies of methylation-disease associations.
Subject: Asthma
Bs-OS-sequencing
CpG sites
DNA methylation
Epigenetic profiling
IL13
ORMDL3
450&#160
k
MICROARRAY
CHILDHOOD
ASTHMA
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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