Root-type ferredoxin-NADP(+) oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses

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Grabsztunowicz , M , Rantala , M , Ivanauskaite , A , Blomster , T , Koskela , M M , Vuorinen , K , Tyystjarvi , E , Burow , M , Overmyer , K , Mähönen , A P & Mulo , P 2021 , ' Root-type ferredoxin-NADP(+) oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses ' , Plant, Cell and Environment , vol. 44 , no. 2 , pp. 548-558 . https://doi.org/10.1111/pce.13932

Title: Root-type ferredoxin-NADP(+) oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses
Author: Grabsztunowicz, Magda; Rantala, Marjaana; Ivanauskaite, Aiste; Blomster, Tiina; Koskela, Minna M.; Vuorinen, Katariina; Tyystjarvi, Esa; Burow, Meike; Overmyer, Kirk; Mähönen, Ari P.; Mulo, Paula
Contributor organization: Helsinki Institute of Life Science HiLIFE
Organismal and Evolutionary Biology Research Programme
Viikki Plant Science Centre (ViPS)
Biosciences
Plant-Fungal Interactions Group
Ari Pekka Mähönen / Principal Investigator
Plant Biology
Date: 2021-02
Language: eng
Number of pages: 11
Belongs to series: Plant, Cell and Environment
ISSN: 0140-7791
DOI: https://doi.org/10.1111/pce.13932
URI: http://hdl.handle.net/10138/338073
Abstract: In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.
Subject: Arabidopsis
ferredoxin&#8208
NADP(+) oxidoreductase
gene expression
low temperature
ozone
plastid
root
stress response
NADP+-OXIDOREDUCTASE
THYLAKOID MEMBRANES
GLUTAMATE SYNTHASE
NITRITE REDUCTION
ELECTRON-TRANSFER
DIFFERENT ORGANS
PLANTS
FNR
PURIFICATION
COMPLEXES
11831 Plant biology
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: acceptedVersion


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