Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens

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http://hdl.handle.net/10138/338492

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Marttinen , E M , Lehtonen , M T , van Gessel , N , Reski , R & Valkonen , J P T 2022 , ' Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens ' , Plant, Cell and Environment , vol. 45 , no. 1 , pp. 220-235 . https://doi.org/10.1111/pce.14194

Title: Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens
Author: Marttinen, Eeva M.; Lehtonen, Mikko T.; van Gessel, Nico; Reski, Ralf; Valkonen, Jari P.T.
Contributor organization: Department of Agricultural Sciences
Plant Production Sciences
Viikki Plant Science Centre (ViPS)
Plant Pathology and Virology
Date: 2022-01
Language: eng
Number of pages: 16
Belongs to series: Plant, Cell and Environment
ISSN: 0140-7791
DOI: https://doi.org/10.1111/pce.14194
URI: http://hdl.handle.net/10138/338492
Abstract: Plant viruses are important pathogens able to overcome plant defense mechanisms using their viral suppressors of RNA silencing (VSR). Small RNA pathways of bryophytes and vascular plants have significant similarities, but little is known about how viruses interact with mosses. This study elucidated the responses of Physcomitrella patens to two different VSRs. We transformed P. patens plants to express VSR P19 from tomato bushy stunt virus and VSR 2b from cucumber mosaic virus, respectively. RNA sequencing and quantitative PCR were used to detect the effects of VSRs on gene expression. Small RNA (sRNA) sequencing was used to estimate the influences of VSRs on the sRNA pool of P. patens. Expression of either VSR-encoding gene caused developmental disorders in P. patens. The transcripts of four different transcription factors (AP2/erf, EREB-11 and two MYBs) accumulated in the P19 lines. sRNA sequencing revealed that VSR P19 significantly changed the microRNA pool in P. patens. Our results suggest that VSR P19 is functional in P. patens and affects the abundance of specific microRNAs interfering with gene expression. The results open new opportunities for using Physcomitrella as an alternative system to study plant-virus interactions.
Subject: 11831 Plant biology
2b
microRNA
moss
P19
Physcomitrium patens
silencing suppressors
VSR
MOSS
CONTROLS LEAF
BLADE-ON-PETIOLE-1
PEROXIDASE
ANTIVIRAL DEFENSE
GENE-EXPRESSION
CELL-CYCLE
INFECTION
ARABIDOPSIS
ARGONAUTES
11832 Microbiology and virology
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: publishedVersion


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