Investigation and Computational Analysis of the Sulfotransferase (SOT) Gene Family in Potato (Solanum tuberosum) : Insights into Sulfur Adjustment for Proper Development and Stimuli Responses

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http://hdl.handle.net/10138/338544

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Faraji , S , Heidari , P , Amouei , H , Filiz , E & Poczai , P 2021 , ' Investigation and Computational Analysis of the Sulfotransferase (SOT) Gene Family in Potato (Solanum tuberosum) : Insights into Sulfur Adjustment for Proper Development and Stimuli Responses ' , Plants (Basel) , vol. 10 , no. 12 , 2597 . https://doi.org/10.3390/plants10122597

Julkaisun nimi: Investigation and Computational Analysis of the Sulfotransferase (SOT) Gene Family in Potato (Solanum tuberosum) : Insights into Sulfur Adjustment for Proper Development and Stimuli Responses
Tekijä: Faraji, Sahar; Heidari, Parviz; Amouei, Hoorieh; Filiz, Ertugrul; Poczai, Peter
Tekijän organisaatio: Botany
Embryophylo
Päiväys: 2021-12
Kieli: eng
Sivumäärä: 22
Kuuluu julkaisusarjaan: Plants (Basel)
ISSN: 2223-7747
DOI-tunniste: https://doi.org/10.3390/plants10122597
URI: http://hdl.handle.net/10138/338544
Tiivistelmä: Various kinds of primary metabolisms in plants are modulated through sulfate metabolism, and sulfotransferases (SOTs), which are engaged in sulfur metabolism, catalyze sulfonation reactions. In this study, a genome-wide approach was utilized for the recognition and characterization of SOT family genes in the significant nutritional crop potato (Solanum tuberosum L.). Twenty-nine putative StSOT genes were identified in the potato genome and were mapped onto the nine S. tuberosum chromosomes. The protein motifs structure revealed two highly conserved 5 '-phosphosulfate-binding (5 ' PSB) regions and a 3 '-phosphate-binding (3 ' PB) motif that are essential for sulfotransferase activities. The protein-protein interaction networks also revealed an interesting interaction between SOTs and other proteins, such as PRTase, APS-kinase, protein phosphatase, and APRs, involved in sulfur compound biosynthesis and the regulation of flavonoid and brassinosteroid metabolic processes. This suggests the importance of sulfotransferases for proper potato growth and development and stress responses. Notably, homology modeling of StSOT proteins and docking analysis of their ligand-binding sites revealed the presence of proline, glycine, serine, and lysine in their active sites. An expression essay of StSOT genes via potato RNA-Seq data suggested engagement of these gene family members in plants' growth and extension and responses to various hormones and biotic or abiotic stimuli. Our predictions may be informative for the functional characterization of the SOT genes in potato and other nutritional crops.
Avainsanat: sulfur
sulfotransferase
potato
bioinformatics
protein structure
stimuli coping
GENOME-WIDE IDENTIFICATION
INDUCIBLE STEROID SULFOTRANSFERASE
DESULFOGLUCOSINOLATE SULFOTRANSFERASE
ARABIDOPSIS-THALIANA
REGULATORY ELEMENTS
BRASSICA-NAPUS
EXPRESSION
WEB
PREDICTION
INFECTION
11831 Plant biology
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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