Single Cell Trapping by Superhydrophobic/Superhydrophilic Microarrays

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Raittinen , P , Elomaa , P , Saavalainen , P & Jokinen , V 2021 , ' Single Cell Trapping by Superhydrophobic/Superhydrophilic Microarrays ' , Advanced Materials Interfaces , vol. 8 , no. 9 , 2100147 .

Title: Single Cell Trapping by Superhydrophobic/Superhydrophilic Microarrays
Author: Raittinen, Paavo; Elomaa, Pinja; Saavalainen, Päivi; Jokinen, Ville
Contributor organization: Research Programs Unit
TRIMM - Translational Immunology Research Program
University of Helsinki
Faculty of Medicine
Department of Medical and Clinical Genetics
Date: 2021-05
Language: eng
Number of pages: 6
Belongs to series: Advanced Materials Interfaces
ISSN: 2196-7350
Abstract: Single cell trapping is demonstrated on superhydrophobic-superhydrophilic patterned microarrays. Superhydrophilic spots that are twice the size of the cells to be trapped are found to be optimal for obtaining single cell trapping. The single cell trapping is based on size exclusivity instead of heavy dilution of the cell suspension and relying on Poisson statistics. The superhydrophobic areas of the array are found to be very resistant toward unwanted adhesion of the cells and thus cleaning steps are not needed after deposition. Based on these properties, 20 mu m superhydrophilic spots are utilized for trapping two types of immune cells, primary peripheral blood mononuclear cells (PBMCs) and THP-1 cells. The cells are trapped from a 10 mu l cell suspension droplet dragged across the surface with different velocities. Single cell trapping efficiencies in the range of 10-30% are shown based on the cell type, the spot type, the seeding velocity and the cell suspension concentration.
Subject: biomaterials
black silicon
single cell analysis
wettability patterning
318 Medical biotechnology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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