Dual Transcriptional Profiling of Carrot and 'Candidatus Liberibacter solanacearum' at Different Stages of Infection Suggests Complex Host-Pathogen Interaction

Show full item record



Permalink

http://hdl.handle.net/10138/340493

Citation

Wang , J , Haapalainen , M , Nissinen , A I & Pirhonen , M 2021 , ' Dual Transcriptional Profiling of Carrot and 'Candidatus Liberibacter solanacearum' at Different Stages of Infection Suggests Complex Host-Pathogen Interaction ' , Molecular Plant - Microbe Interactions , vol. 34 , no. 11 , pp. 1281-1297 . https://doi.org/10.1094/MPMI-10-20-0274-R

Title: Dual Transcriptional Profiling of Carrot and 'Candidatus Liberibacter solanacearum' at Different Stages of Infection Suggests Complex Host-Pathogen Interaction
Author: Wang, Jinhui; Haapalainen, Minna; Nissinen, Anne I.; Pirhonen, Minna
Contributor organization: Faculty of Agriculture and Forestry
Department of Agricultural Sciences
Plant Production Sciences
Viikki Plant Science Centre (ViPS)
Minna Pirhonen / Principal Investigator
Date: 2021-11
Language: eng
Number of pages: 17
Belongs to series: Molecular Plant - Microbe Interactions
ISSN: 0894-0282
DOI: https://doi.org/10.1094/MPMI-10-20-0274-R
URI: http://hdl.handle.net/10138/340493
Abstract: The interactions between the phloem-limited pathogen 'Candidatus Liberibacter solanacearum' haplotype C and carrot (Daucus carota subsp. sativus) were studied at 4, 5, and 9 weeks postinoculation (wpi), by combining dual RNA-Seq results with data on bacterial colonization and observations of the plant phenotype. In the infected plants, genes involved in jasmonate biosynthesis, salicylate signaling, pathogen-associated molecular pattern- and effector-triggered immunity, and production of pathogenesis-related proteins were up-regulated. At 4 wpi, terpenoid synthesis-related genes were up-regulated, presumably as a response to the psyllid feeding, whereas at 5 and 9 wpi, genes involved in both the terpenoid and flavonoid production were down-regulated and phenylpropanoid genes were up-regulated. Chloroplast-related gene expression was down-regulated, in concordance with the observed yellowing of the infected plant leaves. Both the RNA-Seq data and electron microscopy suggested callose accumulation in the infected phloem vessels, likely to impair the transport of photosynthates, while phloem regeneration was suggested by the formation of new sieve cells and the upregulation of cell wall-related gene expression. The 'Ca. L. solanacearum' genes involved in replication, transcription, and translation were expressed at high levels at 4 and 5 wpi, whereas, at 9 wpi, the Flp pilus genes were highly expressed, suggesting adherence and reducedmobility of the bacteria. The 'Ca. L. solanacearum' genes encoding ATP and C4-dicarboxylate uptake were differentially expressed between the early and late infection stages, suggesting a change in the dependence on different host-derived energy sources. HPE1 effector and salicylate hydroxylasewere expressed, presumably to suppress host cell death and salicylic acid-dependent defenses during the infection.
Subject: Daucus carota
dual RNA-Seq
plant hormones
plant-pathogen interactions
Trioza apicalis
DEFENSE RESPONSES
ABSCISIC-ACID
PLANT
GROWTH
ARABIDOPSIS
ASIATICUS
ROLES
POTATO
LEAF
ASSOCIATION
11831 Plant biology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


Files in this item

Total number of downloads: Loading...

Files Size Format View
Carrot.pdf 1.909Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record