Liposome Co-sedimentation and Co-flotation Assays to Study Lipid-Protein Interactions

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Senju , Y , Lappalainen , P & Zhao , H 2021 , Liposome Co-sedimentation and Co-flotation Assays to Study Lipid-Protein Interactions . in RJ Botelho (ed.) , Phosphoinositides . Methods in Molecular Biology , vol. 2251 , Humana press , pp. 195-204 . https://doi.org/10.1007/978-1-0716-1142-5_14

Title: Liposome Co-sedimentation and Co-flotation Assays to Study Lipid-Protein Interactions
Author: Senju, Yosuke; Lappalainen, Pekka; Zhao, Hongxia
Other contributor: Botelho, RJ
Contributor organization: Institute of Biotechnology
Biosciences
Pekka Lappalainen / Principal Investigator
Molecular and Integrative Biosciences Research Programme
Mitochondrial Morphogenesis
Publisher: Humana press
Date: 2021
Language: eng
Number of pages: 10
Belongs to series: Phosphoinositides
Belongs to series: Methods in Molecular Biology
ISBN: 978-1-0716-1141-8
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-0716-1142-5_14
URI: http://hdl.handle.net/10138/340528
Abstract: A large proportion of proteins are expected to interact with cellular membranes to carry out their physiological functions in processes such as membrane transport, morphogenesis, cytoskeletal organization, and signal transduction. The recruitment of proteins at the membrane-cytoplasm interface and their activities are precisely regulated by phosphoinositides, which are negatively charged phospholipids found on the cytoplasmic leaflet of cellular membranes and play critical roles in membrane homeostasis and cellular signaling. Thus, it is important to reveal which proteins interact with phosphoinositides and to elucidate the underlying mechanisms. Here, we present two standard in vitro methods, liposome co-sedimentation and co-flotation assays, to study lipid-protein interactions. Liposomes can mimic various biological membranes in these assays because their lipid compositions and concentrations can be varied. Thus, in addition to mechanisms of lipid-protein interactions, these methods provide information on the possible specificities of proteins toward certain lipids such as specific phosphoinositide species and can hence shed light on the roles of membrane interactions on the functions of membrane-associated proteins.
Subject: Phosphoinositides
Liposome
Lipid-protein interactions
Phospholipids
Binding mode
N-WASP
MEMBRANE
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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