Evaluation of three molecular carbapenemase tests : Eazyplex SuperBug complete B, Novodiag CarbaR plus , and Amplidiag CarbaR plus MCR

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Holma , T , Antikainen , J & Haiko , J 2021 , ' Evaluation of three molecular carbapenemase tests : Eazyplex SuperBug complete B, Novodiag CarbaR plus , and Amplidiag CarbaR plus MCR ' , Journal of Microbiological Methods , vol. 180 , 106105 . https://doi.org/10.1016/j.mimet.2020.106105

Title: Evaluation of three molecular carbapenemase tests : Eazyplex SuperBug complete B, Novodiag CarbaR plus , and Amplidiag CarbaR plus MCR
Author: Holma, Tanja; Antikainen, Jenni; Haiko, Johanna
Contributor organization: HUSLAB
Clinicum
Date: 2021-01
Language: eng
Number of pages: 5
Belongs to series: Journal of Microbiological Methods
ISSN: 0167-7012
DOI: https://doi.org/10.1016/j.mimet.2020.106105
URI: http://hdl.handle.net/10138/341339
Abstract: Background: Carbapenemase-producing Gram-negative bacilli, i.e., Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter, are of increased concern for the public health around the world. There is urgent need for rapid and accurate tests in order to provide correct treatment and to prevent bacterial spread in healthcare settings. Methods: The aim of this study was to evaluate three commercial multiplex carbapenemase tests with CE-IVD marking: Eazyplex SuperBug complete B (AmplexDiagnostics), Novodiag CarbaR+ (Mobidiag), and Amplidiag CarbaR+MCR (Mobidiag). All these tests recognize KPC, NDM, OXA-48/181 group, VIM, OXA-23 group, and OXA-24/40 group, and Novodiag CarbaR+ and Amplidiag CarbaR+MCR additionally recognize IMP, OXA-51 group (with promoter located within ISAbaI), OXA-58 group, and MCR, and Amplidiag CarbaR+MCR further recognizes GES (carbapenemase-type only). Results: The sensitivities and specificities of these tests with bacterial isolates were 100%. The sensitivity directly from clinical samples was 100%, but the specificity was lower, which is simply explained by the higher sensitivity of the molecular methods compared with culture method. Conclusions: Overall, these CE-IVD marked tests provide a good alternative in the detection of carbapenemase-producing organisms.
Subject: CPE
CPO
Carbapenemase
Hospital-acquired infections
Molecular diagnostics
Rapid diagnostics
ENTEROBACTERIACEAE
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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