Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog

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http://hdl.handle.net/10138/341385

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DoGA consortium , Ezer , S , Yoshihara , M , Katayama , S , Daub , C , Lohi , H , Krjutskov , K & Kere , J 2021 , ' Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog ' , STAR protocols , vol. 2 , no. 4 , 100995 . https://doi.org/10.1016/j.xpro.2021.100995

Title: Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog
Author: DoGA consortium; Ezer, Sini; Yoshihara, Masahito; Katayama, Shintaro; Daub, Carsten; Lohi, Hannes; Krjutskov, Kaarel; Kere, Juha
Contributor organization: Research Programs Unit
STEMM - Stem Cells and Metabolism Research Program
Helsinki One Health (HOH)
Haartman Institute (-2014)
Hannes Tapani Lohi / Principal Investigator
Veterinary Genetics
Veterinary Biosciences
Biosciences
Department of Medical and Clinical Genetics
STEMM - Stem Cells and Metabolism Research Program
Juha Kere / Principal Investigator
Date: 2021-12-17
Language: eng
Number of pages: 21
Belongs to series: STAR protocols
ISSN: 2666-1667
DOI: https://doi.org/10.1016/j.xpro.2021.100995
URI: http://hdl.handle.net/10138/341385
Abstract: We have developed a protocol for barcoded cDNA libraries of 48 samples to study gene expression across tissues in the domestic dog, Canis familiaris, by modifying the Single-Cell Tagged Reverse Transcription (STRT) protocol (Islam et al., 2012, 2014). The cDNA reads represent mRNA 5′ ends, enabling the study of transcription start sites (TSS). Our modifications include longer UMIs for molecular counting and Globin-Lock® to deplete globin mRNAs that are abundant in blood and blood-rich tissues dominating all reads.
Description: Publisher Copyright: © 2021 The Authors
Subject: 3111 Biomedicine
1184 Genetics, developmental biology, physiology
413 Veterinary science
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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