The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner

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http://hdl.handle.net/10138/341849

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Toth , K , Lenart , N , Berki , P , Fekete , R , Szabadits , E , Posfai , B , Cserep , C , Alatshan , A , Benko , S , Kiss , D , Huebner , C A , Gulyas , A , Kaila , K , Koernyei , Z & Denes , A 2022 , ' The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner ' , PLoS Biology , vol. 20 , no. 1 , 3001526 . https://doi.org/10.1371/journal.pbio.3001526

Title: The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner
Author: Toth, Krisztina; Lenart, Nikolett; Berki, Peter; Fekete, Rebeka; Szabadits, Eszter; Posfai, Balazs; Cserep, Csaba; Alatshan, Ahmad; Benko, Szilvia; Kiss, Daniel; Huebner, Christian A.; Gulyas, Attila; Kaila, Kai; Koernyei, Zsuzsanna; Denes, Adam
Contributor organization: Molecular and Integrative Biosciences Research Programme
Neuroscience Center
Kai Kaila / Principal Investigator
Laboratory of Neurobiology
Date: 2022-01
Language: eng
Number of pages: 31
Belongs to series: PLoS Biology
ISSN: 1544-9173
DOI: https://doi.org/10.1371/journal.pbio.3001526
URI: http://hdl.handle.net/10138/341849
Abstract: The NKCC1 ion transporter contributes to the pathophysiology of common neurological disorders, but its function in microglia, the main inflammatory cells of the brain, has remained unclear to date. Therefore, we generated a novel transgenic mouse line in which microglial NKCC1 was deleted. We show that microglial NKCC1 shapes both baseline and reactive microglia morphology, process recruitment to the site of injury, and adaptation to changes in cellular volume in a cell-autonomous manner via regulating membrane conductance. In addition, microglial NKCC1 deficiency results in NLRP3 inflammasome priming and increased production of interleukin-1 beta (IL-1 beta), rendering microglia prone to exaggerated inflammatory responses. In line with this, central (intracortical) administration of the NKCC1 blocker, bumetanide, potentiated intracortical lipopolysaccharide (LPS)-induced cytokine levels. In contrast, systemic bumetanide application decreased inflammation in the brain. Microglial NKCC1 KO animals exposed to experimental stroke showed significantly increased brain injury, inflammation, cerebral edema, and, worse, neurological outcome. Thus, NKCC1 emerges as an important player in controlling microglial ion homeostasis and inflammatory responses through which microglia modulate brain injury. The contribution of microglia to central NKCC1 actions is likely to be relevant for common neurological disorders.
Subject: CATION-CHLORIDE COTRANSPORTERS
ACTIVATED CL-CHANNELS
INTERLEUKIN-1
RAMIFICATION
HOMEOSTASIS
SURVEILLANCE
MECHANISMS
BUMETANIDE
PLASTICITY
CONTRIBUTE
3112 Neurosciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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