Osteoblast Attachment on Titanium Coated with Hydroxyapatite by Atomic Layer Deposition

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Kylmaoja , E , Holopainen , J , Abushahba , F , Ritala , M & Tuukkanen , J 2022 , ' Osteoblast Attachment on Titanium Coated with Hydroxyapatite by Atomic Layer Deposition ' , Biomolecules , vol. 12 , no. 5 , 654 . https://doi.org/10.3390/biom12050654

Title: Osteoblast Attachment on Titanium Coated with Hydroxyapatite by Atomic Layer Deposition
Author: Kylmaoja, Elina; Holopainen, Jani; Abushahba, Faleh; Ritala, Mikko; Tuukkanen, Juha
Contributor organization: Helsinki Institute of Sustainability Science (HELSUS)
Forest Economics, Business and Society
Department of Chemistry
Mikko Ritala / Principal Investigator
Doctoral Programme in Chemistry and Molecular Sciences
Doctoral Programme in Materials Research and Nanosciences
Date: 2022-05
Language: eng
Number of pages: 13
Belongs to series: Biomolecules
ISSN: 2218-273X
DOI: https://doi.org/10.3390/biom12050654
URI: http://hdl.handle.net/10138/344443
Abstract: Background: The increasing demand for bone implants with improved osseointegration properties has prompted researchers to develop various coating types for metal implants. Atomic layer deposition (ALD) is a method for producing nanoscale coatings conformally on complex three-dimensional surfaces. We have prepared hydroxyapatite (HA) coating on titanium (Ti) substrate with the ALD method and analyzed the biocompatibility of this coating in terms of cell adhesion and viability. Methods: HA coatings were prepared on Ti substrates by depositing CaCO3 films by ALD and converting them to HA by wet treatment in dilute phosphate solution. MC3T3-E1 preosteoblasts were cultured on ALD-HA, glass slides and bovine bone slices. ALD-HA and glass slides were either coated or non-coated with fibronectin. After 48h culture, cells were imaged with scanning electron microscopy (SEM) and analyzed by vinculin antibody staining for focal adhesion localization. An 344,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test was performed to study cell viability. Results: Vinculin staining revealed similar focal adhesion-like structures on ALD-HA as on glass slides and bone, albeit on ALD-HA and bone the structures were thinner compared to glass slides. This might be due to thin and broad focal adhesions on complex three-dimensional surfaces of ALD-HA and bone. The MTT test showed comparable cell viability on ALD-HA, glass slides and bone. Conclusion: ALD-HA coating was shown to be biocompatible in regard to cell adhesion and viability. This leads to new opportunities in developing improved implant coatings for better osseointegration and implant survival.
Subject: atomic layer deposition
hydroxyapatite
titanium
osteoblast
MC3T3
BETA-TRICALCIUM PHOSPHATE
SURFACE-ROUGHNESS
MC3T3-E1 CELLS
CERAMIC COATINGS
BONE-GROWTH
THIN-FILMS
IN-VIVO
ADHESION
IMPLANT
DIFFERENTIATION
116 Chemical sciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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