Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells

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http://hdl.handle.net/10138/345523

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Capra , J , Härkönen , K , Kyykallio , H , Vihinen , H , Jokitalo , E & Rilla , K 2022 , ' Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells ' , European Journal of Cell Biology , vol. 101 , no. 3 , 151235 . https://doi.org/10.1016/j.ejcb.2022.151235

Title: Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells
Author: Capra, Janne; Härkönen, Kai; Kyykallio, Heikki; Vihinen, Helena; Jokitalo, Eija; Rilla, Kirsi
Contributor organization: Institute of Biotechnology
Electron Microscopy
Biosciences
Date: 2022-08
Language: eng
Number of pages: 10
Belongs to series: European Journal of Cell Biology
ISSN: 0171-9335
DOI: https://doi.org/10.1016/j.ejcb.2022.151235
URI: http://hdl.handle.net/10138/345523
Abstract: We have shown the connection of hyaluronan synthesis activity with the enhanced shedding of extracellular vesicles, but detailed morphological analysis of those hyaluronan-induced EVs is still missing. In this study we utilized a comprehensive set of high-resolution imaging techniques to characterize in high detail the size and morphology of EVs originating from stable MCF7 breast cancer cell line and transiently transfected cells expressing GFP-HAS3. To avoid possible artefacts or loss of EVs resulting from the isolation process, special attention was paid to analysis of EVs in situ in monolayer and in 3D cultures. The results of this study show that GFP-HAS3 expressing MCF7 cells produce morphologically diverse EVs but also demonstrates the variation in results obtained with different experimental setup, which emphasizes the importance of comparison between different methods when interpreting the observations.
Subject: Extracellular vesicle
Morphology
Hyaluronan synthase
Confocal microscopy
3D culture
Electron tomography
EXTRACELLULAR VESICLES
HYALURONAN SYNTHESIS
EXOSOMES
MICROVESICLES
SYNTHASE
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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