How water-soluble saccharides drive the metabolism of lactic acid bacteria during fermentation of brewers' spent grain

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Acin-Albiac , M , Filannino , P , Coda , R , Rizzello , C G , Gobbetti , M & Di Cagno , R 2022 , ' How water-soluble saccharides drive the metabolism of lactic acid bacteria during fermentation of brewers' spent grain ' , Microbial Biotechnology , vol. 15 , no. 3 , pp. 915-930 . https://doi.org/10.1111/1751-7915.13846

Title: How water-soluble saccharides drive the metabolism of lactic acid bacteria during fermentation of brewers' spent grain
Author: Acin-Albiac, Marta; Filannino, Pasquale; Coda, Rossana; Rizzello, Carlo Giuseppe; Gobbetti, Marco; Di Cagno, Raffaella
Contributor organization: Department of Food and Nutrition
Helsinki Institute of Sustainability Science (HELSUS)
Grain Technology
Date: 2022-03
Language: eng
Number of pages: 16
Belongs to series: Microbial Biotechnology
ISSN: 0964-7562
DOI: https://doi.org/10.1111/1751-7915.13846
URI: http://hdl.handle.net/10138/346059
Abstract: We proposed a novel phenomic approach to track the effect of short-term exposures of Lactiplantibacillus plantarum and Leuconostoc pseudomesenteroides to environmental pressure induced by brewers' spent grain (BSG)-derived saccharides. Water-soluble BSG-based medium (WS-BSG) was chosen as model system. The environmental pressure exerted by WS-BSG shifted the phenotypes of bacteria in species- and strains-dependent way. The metabolic drift was growth phase-dependent and likely underlay the diauxic profile of organic acids production by bacteria in response to the low availability of energy sources. Among pentosans, metabolism of arabinose was preferred by L. plantarum and xylose by Leuc. pseudomesenteroides as confirmed by the overexpression of related genes. Bayesian variance analysis showed that phenotype switching towards galactose metabolism suffered the greatest fluctuation in L. plantarum. All lactic acid bacteria strains utilized more intensively sucrose and its plant-derived isomers. Sucrose-6-phosphate activity in Leuc. pseudomesenteroides likely mediated the increased consumption of raffinose. The increased levels of some phenolic compounds suggested the involvement of 6-phospho-beta-glucosidases in beta-glucosides degradation. Expression of genes encoding beta-glucoside/cellobiose-specific EII complexes and phenotyping highlighted an increased metabolism for cellobiose. Our reconstructed metabolic network will improve the understanding of how lactic acid bacteria may transform BSG into suitable food ingredients.
Subject: 416 Food Science
220 Industrial biotechnology
LACTOBACILLUS-PLANTARUM
PERFORMANCE
EXPRESSION
PHENOLICS
TRANSPORT
SOFTWARE
STRAINS
GROWTH
FOOD
TOOL
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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