In-depth characterization of denitrifier communities across different soil ecosystems in the tundra

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http://urn.fi/URN:NBN:fi-fe2022071151626 http://hdl.handle.net/10138/346111

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Title: In-depth characterization of denitrifier communities across different soil ecosystems in the tundra
Author: Pessi, Igor S.; Viitamäki, Sirja; Virkkala, Anna-Maria; Eronen-Rasimus, Eeva; Delmont, Tom O.; Marushchak, Maija E.; Luoto, Miska; Hultman, Jenni
Contributor organization: Suomen ympäristökeskus
The Finnish Environment Institute
Publisher: Springer Science and Business Media LLC
Date: 2022
Language: en
Belongs to series: Environmental Microbiome
ISSN: 2524-6372
DOI: https://doi.org/10.1186/s40793-022-00424-2
URI: http://urn.fi/URN:NBN:fi-fe2022071151626
http://hdl.handle.net/10138/346111
Abstract: Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are variable sources and sinks of N2O, we aimed at investigating differences in community structure across different soil ecosystems in the tundra. Results We analysed 1.4 Tb of metagenomic data from soils in northern Finland covering a range of ecosystems from dry upland soils to water-logged fens and obtained 796 manually binned and curated metagenome-assembled genomes (MAGs). We then searched for MAGs harbouring genes involved in denitrification, an important process driving N2O emissions. Communities of potential denitrifiers were dominated by microorganisms with truncated denitrification pathways (i.e., lacking one or more denitrification genes) and differed across soil ecosystems. Upland soils showed a strong N2O sink potential and were dominated by members of the Alphaproteobacteria such as Bradyrhizobium and Reyranella. Fens, which had in general net-zero N2O fluxes, had a high abundance of poorly characterized taxa affiliated with the Chloroflexota lineage Ellin6529 and the Acidobacteriota subdivision Gp23. Conclusions By coupling an in-depth characterization of microbial communities with in situ measurements of N2O fluxes, our results suggest that the observed spatial patterns of N2O fluxes in the tundra are related to differences in the composition of denitrifier communities.
Subject: nitrous oxide;
metagenomics;
arctic region
denitrification
soil microbiome
Arctic
Genome-resolved metagenomics
maaperä
ekosysteemit (ekologia)
arktinen alue
tundra
kasvihuonekaasut
dityppioksidi
typpi
nitraatit
denitrifikaatio
Rights: CC BY 4.0


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