Cytochrome P450 Inhibition by Antimicrobials and Their Mixtures in Rainbow Trout Liver Microsomes In Vitro

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http://hdl.handle.net/10138/346140

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Pihlaja , T L M , Niemissalo , S M & Sikanen , T M 2022 , ' Cytochrome P450 Inhibition by Antimicrobials and Their Mixtures in Rainbow Trout Liver Microsomes In Vitro ' , Environmental Toxicology and Chemistry , vol. 41 , no. 3 , pp. 663-676 . https://doi.org/10.1002/etc.5160

Title: Cytochrome P450 Inhibition by Antimicrobials and Their Mixtures in Rainbow Trout Liver Microsomes In Vitro
Author: Pihlaja, Tea L. M.; Niemissalo, Sanna M.; Sikanen, Tiina M.
Contributor organization: Tiina Sikanen / Chemical Microsystems Lab
Drug Research Program
Faculty of Pharmacy
Date: 2022-03
Language: eng
Number of pages: 14
Belongs to series: Environmental Toxicology and Chemistry
ISSN: 0730-7268
DOI: https://doi.org/10.1002/etc.5160
URI: http://hdl.handle.net/10138/346140
Abstract: Antimicrobials are ubiquitous in the environment and can bioaccumulate in fish. In the present study, we determined the half-maximal inhibitory concentrations (IC50) of 7 environmentally abundant antimicrobials (ciprofloxacin, clarithromycin, clotrimazole, erythromycin, ketoconazole, miconazole, and sulfamethoxazole) on the cytochrome P450 (CYP) system in rainbow trout (Oncorhynchus mykiss) liver microsomes, using 7-ethoxyresorufin O-deethylation (EROD, CYP1A) and 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylation (BFCOD, CYP3A) as model reactions. Apart from ciprofloxacin and sulfamethoxazole, all antimicrobials inhibited either EROD or BFCOD activities or both at concentrations
Subject: BIOCONCENTRATION
Bioaccumulation
CRUCIAN CARP
CYP3A
Cytochrome P450
ERYTHROMYCIN
Environmental risk assessment
FISH
KETOCONAZOLE
METABOLISM
Mixtures
PHARMACEUTICALS
Pharmaceuticals
TIME-DEPENDENT INHIBITION
TISSUE DISTRIBUTION
Xenobiotic metabolism
1172 Environmental sciences
317 Pharmacy
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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