SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces

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Haliloglu , K , Turkoglu , A , Tan , M & Poczai , P 2022 , ' SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces ' , Genes , vol. 13 , no. 6 , 1086 . https://doi.org/10.3390/genes13061086

Title: SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces
Author: Haliloglu, Kamil; Turkoglu, Aras; Tan, Mustafa; Poczai, Peter
Contributor organization: Viikki Plant Science Centre (ViPS)
Botany
Embryophylo
Finnish Museum of Natural History
Date: 2022-06
Language: eng
Number of pages: 16
Belongs to series: Genes
ISSN: 2073-4425
DOI: https://doi.org/10.3390/genes13061086
URI: http://hdl.handle.net/10138/346235
Abstract: Plant genetic diversity has a significant role in providing traits that can help meet future challenges, such as the need to adapt crops to changing climatic conditions or outbreaks of disease. Our aim in this study was to evaluate the diversity of 61 forage pea specimens (P. sativum ssp. arvense L.) collected from the northeastern Anatolia region of Turkey using 28 simple sequence repeat (SSR) markers. These primers generated a total of 82 polymorphic bands. The number of observed alleles (Na) per primer varied from 2 to 4 with a mean of 2.89 alleles/locus. The mean value of expected heterozygosity (Exp-Het = 0.50) was higher than the mean value of observed heterozygosity (Obs-Het = 0.22). The mean of polymorphic information content (PIC) was 0.41 with a range of 0.03-0.70. The mean number of effective alleles (Ne) was found to be 2.15, Nei's expected heterozygosity (H) 0.49, and Shannon's information index (I) 0.81. Cluster analysis through the unweighted pair-group mean average (UPGMA) method revealed that 61 forage pea landraces were divided into three main clusters. Genetic dissimilarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 0.10 (G30 and G34) and 0.66 (G1 and G32). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 51.54% of the total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2669 (the first sub-population) and 0.3223 (third sub-population), with an average value of 0.2924. Average population differentiation measurement (Fst) was identified as 0.2351 for the first sub-population, 0.3838 for the second sub-population, and 0.2506 for the third sub-population. In general, current results suggest that SSR markers could be constantly used to illuminate the genetic diversity of forage pea landraces and can potentially be incorporated into future studies that examine the diversity within a larger collection of forage pea genotypes from diverse regions.
Subject: genetic
molecular markers
structure
UPGMA
WILD-SPECIES ACCESSIONS
GENETIC DIVERSITY
MORPHOLOGICAL TRAITS
MICROSATELLITE
VARIETIES
RETROTRANSPOSON
ASSOCIATION
GERMPLASM
GENOTYPES
MARKERS
11831 Plant biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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