Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya

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Masika , M M , Korhonen , E M , Smura , T , Uusitalo , R , Ogola , J , Mwaengo , D , Jääskeläinen , A J , Alburkat , H , Gwon , Y-D , Evander , M , Anzala , O , Vapalahti , O & Huhtamo , E 2022 , ' Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya ' , Viruses (Basel) , vol. 14 , no. 6 , 1286 . https://doi.org/10.3390/v14061286

Title: Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya
Author: Masika, Moses Muia; Korhonen, Essi M.; Smura, Teemu; Uusitalo, Ruut; Ogola, Joseph; Mwaengo, Dufton; Jääskeläinen, Anne J.; Alburkat, Hussein; Gwon, Yong-Dae; Evander, Magnus; Anzala, Omu; Vapalahti, Olli; Huhtamo, Eili
Contributor organization: Faculty Common Matters (Faculty of Medicine)
Helsinki One Health (HOH)
Viral Zoonosis Research Unit
Department of Virology
University of Helsinki
Veterinary Biosciences
HUSLAB
HUS Diagnostic Center
Department of Geosciences and Geography
Medicum
Emerging Infections Research Group
Veterinary Microbiology and Epidemiology
Olli Pekka Vapalahti / Principal Investigator
Date: 2022-06
Language: eng
Number of pages: 12
Belongs to series: Viruses (Basel)
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v14061286
URI: http://hdl.handle.net/10138/346387
Abstract: Several alphaviruses, such as chikungunya (CHIKV) and Onyong-nyong (ONNV), are endemic in Kenya and often cause outbreaks in different parts of the country. We assessed the seroprevalence of alphaviruses in patients with acute febrile illness in two geographically distant areas in Kenya with no previous record of alphavirus outbreaks. Blood samples were collected from febrile patients in health facilities located in the rural Taita-Taveta County in 2016 and urban Kibera informal settlement in Nairobi in 2017 and tested for CHIKV IgG and IgM antibodies using an in-house immunofluorescence assay (IFA) and a commercial ELISA test, respectively. A subset of CHIKV IgG or IgM antibody-positive samples were further analyzed using plaque reduction neutralization tests (PRNT) for CHIKV, ONNV, and Sindbis virus. Out of 537 patients, 4 (0.7%) and 28 (5.2%) had alphavirus IgM and IgG antibodies, respectively, confirmed on PRNT. We show evidence of previous and current exposure to alphaviruses based on serological testing in areas with no recorded history of outbreaks.
Subject: arbovirus
alphavirus
chikungunya
Onyong-nyong
seroprevalence
ELISA
neutralization
immunofluorescence
febrile illness
ARBOVIRUSES
FEVER
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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