Activation of NLRP3 Inflammasome in the Skin of Patients with Systemic and Cutaneous Lupus Erythematosus

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Mähönen , K , Hau , A , Bondet , V , Duffy , D , Eklund , K K , Panelius , J & Ranki , A 2022 , ' Activation of NLRP3 Inflammasome in the Skin of Patients with Systemic and Cutaneous Lupus Erythematosus ' , Acta Dermato-Venereologica , vol. 102 , 00708 . https://doi.org/10.2340/actadv.v102.2293

Title: Activation of NLRP3 Inflammasome in the Skin of Patients with Systemic and Cutaneous Lupus Erythematosus
Author: Mähönen, Katariina; Hau, Annika; Bondet, Vincent; Duffy, Darragh; Eklund, Kari K.; Panelius, Jaana; Ranki, Annamari
Contributor organization: HUS Inflammation Center
Department of Dermatology, Allergology and Venereology
University of Helsinki
Department of Medicine
Clinicum
Reumatologian yksikkö
Date: 2022-05
Language: eng
Number of pages: 8
Belongs to series: Acta Dermato-Venereologica
ISSN: 0001-5555
DOI: https://doi.org/10.2340/actadv.v102.2293
URI: http://hdl.handle.net/10138/346463
Abstract: NLRP3 inflammasome is suggested to contribute to the complex pathogenesis of systemic lupus erythematosus, but its role in cutaneous lupus erythematosus has not been addressed. This study investigated the expression of NLRP3 inflammasome components and levels of type I interferons in the skin of 20 patients with cutaneous lupus erythematosus. Expression of NLRP1/3, adaptor protein ASC (apoptosis-associated speck-like protein), caspase-1, interferon-alpha (IFN-alpha), myxovirus resistance protein (MxA), and interferon-induced proteins 1 and 2 (IFIT 1/2) in the skin was assessed using reverse transcription quantitative real-time PCR (RT-qPCR), western blotting and immunohistochemistry. Serum interferon-a protein levels from 12 patients were measured using digital enzyme-linked immunoassay (ELISA). Interleukin-1 beta expression was significantly upregulated in the lesional skin of patients with cutaneous lupus erythematosus compared with their uninvolved skin. However, NLRP1/3, ASC and caspase-1 were not significantly upregulated compared with the skin of control persons. IFN-alpha and IFN-induced proteins MxA and IFIT1/2 were strongly expressed in cutaneous lupus erythematosus skin. Variability in the expression of NLRP3 inflammasome components among patients suggests heterogeneity of pathological pathways in cutaneous lupus erythematosus.
Subject: cutaneous lupus erythematosus
inflammasomes
IL-1 beta
type I interferon
PATHOGENESIS
INTERLEUKIN-1-BETA
DISEASE
MECHANISM
SPECTRUM
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by_nc
Usage restriction: openAccess
Self-archived version: publishedVersion


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