Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples

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Lahdentausta , L , Kivimäki , A , Oksanen , L , Tallgren , M , Oksanen , S , Sanmark , E , Salminen , A , Geneid , A , Sairanen , M , Paju , S , Saksela , K , Pussinen , P & Pietiäinen , M 2022 , ' Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples ' , Medical Microbiology and Immunology , vol. 211 , no. 4 , pp. 173-183 .

Title: Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples
Author: Lahdentausta, Laura; Kivimäki, Anne; Oksanen, Lotta; Tallgren, Marika; Oksanen, Sampo; Sanmark, Enni; Salminen, Aino; Geneid, Ahmed; Sairanen, Mikko; Paju, Susanna; Saksela, Kalle; Pussinen, Pirkko; Pietiäinen, Milla
Contributor organization: Department of Oral and Maxillofacial Diseases
Faculty of Medicine
University of Helsinki
HUS Head and Neck Center
Department of Ophthalmology and Otorhinolaryngology
Korva-, nenä- ja kurkkutautien klinikka
Faculty Common Matters (Faculty of Medicine)
Department of Virology
Kalle Saksela / Principal Investigator
Infection Biology Research Program
Date: 2022-08
Language: eng
Number of pages: 11
Belongs to series: Medical Microbiology and Immunology
ISSN: 0300-8584
Abstract: We examined the usefulness of dried spot blood and saliva samples in SARS-CoV-2 antibody analyses. We analyzed 1231 self-collected dried spot blood and saliva samples from healthcare workers. Participants filled in a questionnaire on their COVID-19 exposures, infections, and vaccinations. Anti-SARS-CoV-2 IgG, IgA, and IgM levels were determined from both samples using the GSP/DELFIA method. The level of exposure was the strongest determinant of all blood antibody classes and saliva IgG, increasing as follows: (1) no exposure (healthy, non-vaccinated), (2) exposed, (3) former COVID-19 infection, (4) one vaccination, (5) two vaccinations, and (6) vaccination and former infection. While the blood IgG assay had a 99.5% sensitivity and 75.3% specificity to distinguish participants with two vaccinations from all other types of exposure, the corresponding percentages for saliva IgG were 85.3% and 65.7%. Both blood and saliva IgG-seropositivity proportions followed similar trends to the exposures reported in the questionnaires. Self-collected dry blood and saliva spot samples combined with the GSP/DELFIA technique comprise a valuable tool to investigate an individual's immune response to SARS-CoV-2 exposure or vaccination. Saliva IgG has high potential to monitor vaccination response wane, since the sample is non-invasive and easy to collect.
Subject: SARS-CoV-2
Dried spot sample
3111 Biomedicine
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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