Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator

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Matlashov , M E , Vera , J , Kasatkina , L A , Khodakhah , K & Verkhusha , V V 2022 , ' Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator ' , Frontiers in Cell and Developmental Biology , vol. 10 , 880107 . https://doi.org/10.3389/fcell.2022.880107

Title: Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator
Author: Matlashov, Mikhail E.; Vera, Jorge; Kasatkina, Ludmila A.; Khodakhah, Kamran; Verkhusha, Vladislav V.
Contributor organization: Department of Anatomy
University of Helsinki
Medicum
Date: 2022-06-29
Language: eng
Number of pages: 11
Belongs to series: Frontiers in Cell and Developmental Biology
ISSN: 2296-634X
DOI: https://doi.org/10.3389/fcell.2022.880107
URI: http://hdl.handle.net/10138/346595
Abstract: Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs.
Subject: GECI
iRFP
FRET
calcium imaging
far-red
biosensor
PROTEINS
NEURONS
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
1184 Genetics, developmental biology, physiology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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