Interrelationship of the soft rot phytopathogens in the Pectobacterium genus and Caenorhabditis elegans

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dc.contributor Helsingin yliopisto, maatalous-metsätieteellinen tiedekunta, maataloustieteiden laitos fi
dc.contributor University of Helsinki, Faculty of Agriculture and Forestry, Department of Agricultural Sciences en
dc.contributor Helsingfors universitet, agrikultur-forstvetenskapliga fakulteten, institutionen för lantsbruksvetenskaper sv
dc.contributor.author Fang, Xin
dc.date.accessioned 2012-07-10T08:02:04Z
dc.date.accessioned 2015-07-23T07:56:41Z
dc.date.available 2012-07-10T08:02:04Z
dc.date.available 2015-07-23T07:56:41Z
dc.date.issued 2012
dc.identifier.uri URN:NBN:fi:hulib-201507211936
dc.identifier.uri http://hdl.handle.net/10138/35109
dc.description.abstract Bacterial soft rot is one of the most serious diseases affecting potatoes in fields and storages worldwide. Soft rot on potatoes and other crops are caused mainly by Pectobacterium atrosepticum, Pectobacterium carotovorum and several Dickeya species. The disease spreads via many ways and insects are suggested to be important agents in the epidemiology. Evolutionary and geological evidences suggest soil nematodes play essential roles in ecological processes including nutrients cycling, decomposition and disease suppression. Although some studies show that Pectobacteria can survive the digestive tract of the nematodes, it is unclear whether the soil nematodes can be vectors for transmission and dissemination of plant pathogenic bacteria. Moreover, the life span of C. elegans, a free-living and bacterial-feeding nematode, is limited when fed on Pectobacterium. How soft rot bacteria interact with C. elegans and how the nematodes respond to these bacteria are still unknown. Results of this study suggest that Pectobacterium could be disseminated via nematodes on potatoes, indicating that nematodes could serve as vectors in the dissemination of soft rot bacteria on crops. Through the bacterial transmission assay, Pectobacterium is proved to possess a better ability to persist with nematodes compared to E. coli. It seems that pectobacteria have some traits that provide this ability, so Pectobacterium mutants related to Pectobacterium-plant interaction were tested and the expressions C. elegans genes were measured using quantitative polymerase chain reaction (qPCR), aiming to find out genes playing a role in Pectobacterium-nematodes interaction. However, in this study most likely type VI secretion system, putative Flp/Tad-like pilus, flagella, full length lipopolysaccharides, quorum sensing or ExpAS two-component system are not necessary determinants for the interrelationship of Pectobacterium and nematodes. en
dc.language.iso eng
dc.publisher Helsingfors universitet sv
dc.publisher University of Helsinki en
dc.publisher Helsingin yliopisto fi
dc.subject Pectobacterium en
dc.subject nematodes en
dc.subject potato soft rot en
dc.subject vector en
dc.subject interrelationship en
dc.title Interrelationship of the soft rot phytopathogens in the Pectobacterium genus and Caenorhabditis elegans en
dc.type opinnäytteet fi
dc.type Thesis en
dc.type lärdomsprov sv
dc.type.ontasot pro gradu-avhandlingar sv
dc.type.ontasot pro gradu -tutkielmat fi
dc.type.ontasot master's thesis en
dc.subject.discipline Kasvipatologia fi
dct.identifier.urn URN:NBN:fi:hulib-201507211936

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