Cross-species testing and utility of microsatellite loci in Indirana frogs

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Nair , A , Gopalan , S V , George , S , Kumar , K S & Merilä , J 2012 , ' Cross-species testing and utility of microsatellite loci in Indirana frogs ' , BMC research notes , vol. 5 , no. 389 .

Title: Cross-species testing and utility of microsatellite loci in Indirana frogs
Author: Nair, Abhilash; Gopalan, Sujith V.; George, Sanil; Kumar, K. Santhosh; Merilä, Juha
Contributor organization: Ecology and Evolutionary Biology
Ecological Genetics Research Unit
Date: 2012-07
Language: eng
Number of pages: 5
Belongs to series: BMC research notes
ISSN: 1756-0500
Abstract: Background Microsatellite loci are widely used in population and conservation genetic studies of amphibians, but the availability of such markers for tropical and subtropical taxa is currently very limited. In order to develop resources for conservation genetic studies in the genus Indirana, we tested amplification success and polymorphism in 62 previously developed microsatellite loci, in eight Indirana species - including new candidate species. Developing genomic resources for this amphibian taxon is particularly important as it is endemic to the Western Ghats biodiversity hotspot, and harbours several endangered species. Findings The cross-species amplification success rate varied from 11.3 % to 29.0 % depending on the species, with 29 - 80 % of the amplifying loci being polymorphic. A strong negative correlation between cross-species amplification success (and polymorphism) and genetic distance separating target from source species was observed. Conclusions Our results provide additional genetic support for the existence of genetically divergent cryptic species within the genus Indirana. The tested markers should be useful for population and conservation genetic studies in this genus, and in particular, for species closely related to the source species, I. beddomii.
Subject: 1181 Ecology, evolutionary biology
Biodiversity hotspot
Western Ghats
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion

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