Cross-species testing and utility of microsatellite loci in Indirana frogs

Näytä kaikki kuvailutiedot



Pysyväisosoite

http://hdl.handle.net/10138/39164

Lähdeviite

Nair , A , Gopalan , S V , George , S , Kumar , K S & Merilä , J 2012 , ' Cross-species testing and utility of microsatellite loci in Indirana frogs ' , BMC research notes , vol. 5 , no. 389 . https://doi.org/10.1186/1756-0500-5-389

Julkaisun nimi: Cross-species testing and utility of microsatellite loci in Indirana frogs
Tekijä: Nair, Abhilash; Gopalan, Sujith V.; George, Sanil; Kumar, K. Santhosh; Merilä, Juha
Muu tekijä: University of Helsinki, Ecology and Evolutionary Biology
University of Helsinki, Biosciences
Päiväys: 2012-07
Kieli: eng
Sivumäärä: 5
Kuuluu julkaisusarjaan: BMC research notes
ISSN: 1756-0500
URI: http://hdl.handle.net/10138/39164
Tiivistelmä: Background Microsatellite loci are widely used in population and conservation genetic studies of amphibians, but the availability of such markers for tropical and subtropical taxa is currently very limited. In order to develop resources for conservation genetic studies in the genus Indirana, we tested amplification success and polymorphism in 62 previously developed microsatellite loci, in eight Indirana species - including new candidate species. Developing genomic resources for this amphibian taxon is particularly important as it is endemic to the Western Ghats biodiversity hotspot, and harbours several endangered species. Findings The cross-species amplification success rate varied from 11.3 % to 29.0 % depending on the species, with 29 - 80 % of the amplifying loci being polymorphic. A strong negative correlation between cross-species amplification success (and polymorphism) and genetic distance separating target from source species was observed. Conclusions Our results provide additional genetic support for the existence of genetically divergent cryptic species within the genus Indirana. The tested markers should be useful for population and conservation genetic studies in this genus, and in particular, for species closely related to the source species, I. beddomii.
Avainsanat: 1181 Ecology, evolutionary biology
Amphibia
Microsatellite
Indirana
Biodiversity hotspot
Ranixalidae
Western Ghats
Tekijänoikeustiedot:


Tiedostot

Latausmäärä yhteensä: Ladataan...

Tiedosto(t) Koko Formaatti Näytä
Nair_BMC_research_notes_2012.pdf 351.4KB PDF Avaa tiedosto

Viite kuuluu kokoelmiin:

Näytä kaikki kuvailutiedot