Cross-species testing and utility of microsatellite loci in Indirana frogs

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dc.contributor University of Helsinki, Ecology and Evolutionary Biology en
dc.contributor University of Helsinki, Biosciences en
dc.contributor.author Nair, Abhilash
dc.contributor.author Gopalan, Sujith V.
dc.contributor.author George, Sanil
dc.contributor.author Kumar, K. Santhosh
dc.contributor.author Merilä, Juha
dc.date.accessioned 2013-05-15T20:01:30Z
dc.date.available 2013-05-15T20:01:30Z
dc.date.issued 2012-07
dc.identifier.citation Nair , A , Gopalan , S V , George , S , Kumar , K S & Merilä , J 2012 , ' Cross-species testing and utility of microsatellite loci in Indirana frogs ' , BMC research notes , vol. 5 , no. 389 . https://doi.org/10.1186/1756-0500-5-389 en
dc.identifier.issn 1756-0500
dc.identifier.other PURE: 25521870
dc.identifier.other PURE UUID: 8e452c1f-666a-483e-9f5a-52bb7de10145
dc.identifier.other Scopus: 84864214861
dc.identifier.other ORCID: /0000-0001-9614-0072/work/29056203
dc.identifier.uri http://hdl.handle.net/10138/39164
dc.description.abstract Background Microsatellite loci are widely used in population and conservation genetic studies of amphibians, but the availability of such markers for tropical and subtropical taxa is currently very limited. In order to develop resources for conservation genetic studies in the genus Indirana, we tested amplification success and polymorphism in 62 previously developed microsatellite loci, in eight Indirana species - including new candidate species. Developing genomic resources for this amphibian taxon is particularly important as it is endemic to the Western Ghats biodiversity hotspot, and harbours several endangered species. Findings The cross-species amplification success rate varied from 11.3 % to 29.0 % depending on the species, with 29 - 80 % of the amplifying loci being polymorphic. A strong negative correlation between cross-species amplification success (and polymorphism) and genetic distance separating target from source species was observed. Conclusions Our results provide additional genetic support for the existence of genetically divergent cryptic species within the genus Indirana. The tested markers should be useful for population and conservation genetic studies in this genus, and in particular, for species closely related to the source species, I. beddomii. en
dc.format.extent 5
dc.language.iso eng
dc.relation.ispartof BMC research notes
dc.rights en
dc.subject 1181 Ecology, evolutionary biology en
dc.subject Amphibia en
dc.subject Microsatellite en
dc.subject Indirana en
dc.subject Biodiversity hotspot en
dc.subject Ranixalidae en
dc.subject Western Ghats en
dc.title Cross-species testing and utility of microsatellite loci in Indirana frogs en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1186/1756-0500-5-389
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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