T-helper Cell-Mediated Proliferation and Cytokine Responses against Recombinant Merkel Cell Polyomavirus-Like Particles

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Kumar , A , Chen , T , Kantele , A , Söderlund-Venermo , M , Hedman , K , Franssila , R & Pakkanen , S H 2011 , ' T-helper Cell-Mediated Proliferation and Cytokine Responses against Recombinant Merkel Cell Polyomavirus-Like Particles ' , PLoS One , vol. 6 , no. 10 , pp. Article Number: e25751 . https://doi.org/10.1371/journal.pone.0025751

Title: T-helper Cell-Mediated Proliferation and Cytokine Responses against Recombinant Merkel Cell Polyomavirus-Like Particles
Author: Kumar, Arun; Chen, Tingting; Kantele, Anu; Söderlund-Venermo, Maria; Hedman, Klaus; Franssila, Rauli; Pakkanen, Sari Hannele
Contributor organization: Department of Virology
Department of Bacteriology and Immunology
Department of Medicine
Infektiosairauksien yksikkö
Haartman Institute (-2014)
Anu Kantele-Häkkinen Research Group
Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact
Virus infections and immunity
Date: 2011
Language: eng
Number of pages: 7
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0025751
URI: http://hdl.handle.net/10138/41614
Abstract: The newly discovered Merkel Cell Polyomavirus (MCPyV) resides in approximately 80% of Merkel cell carcinomas (MCC). Causal role of MCPyV for this rare and aggressive skin cancer is suggested by monoclonal integration and truncation of large T (LT) viral antigen in MCC cells. The mutated MCPyV has recently been found in highly purified leukemic cells from patients with chronic lymphocytic leukemia (CLL), suggesting a pathogenic role also in CLL. About 50–80% of adults display MCPyVspecific antibodies. The humoral immunity does not protect against the development of MCC, as neutralizing MCPyV antibodies occur in higher levels among MCC patients than healthy controls. Impaired T-cell immunity has been linked with aggressive MCC behavior. Therefore, cellular immunity appears to be important in MCPyV infection surveillance. In order to elucidate the role of MCPyV-specific Th-cell immunity, peripheral blood mononuclear cells (PBMC) of healthy adults were stimulated with MCPyV VP1 virus-like particles (VLPs), using human bocavirus (HBoV) VLPs and Candida albicans antigen as positive controls. Proliferation, IFN-c, IL-13 and IL-10 responses were examined in 15 MCPyV-seropositive and 15 seronegative volunteers. With the MCPyV antigen, significantly stronger Th-cell responses were found in MCPyVseropositive than MCPyV-seronegative subjects, whereas with the control antigens, the responses were statistically similar. The most readily detectable cytokine was IFN-c. The MCPyV antigen tended to induce stronger IFN-c responses than HBoV VLP antigen. Taken together, MCPyV-specific Th-cells elicit vigorous IFN-c responses. IFN-c being a cytokine with major antiviral and tumor suppressing functions, Th-cells are suggested to be important mediators of MCPyV-specific immune surveillance.
Subject: CHRONIC LYMPHOCYTIC-LEUKEMIA
INTERFERON-GAMMA
IFN-GAMMA
IMMUNE-RESPONSE
TUMOR-GROWTH
B-CELLS
CARCINOMA
INTERLEUKIN-10
ANTIBODIES
CANCER
3111 Biomedicine
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion


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