Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae)

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dc.contributor.author Poczai, Peter
dc.contributor.author Cernak, Istvan
dc.contributor.author Gorji, Ahmad Mosapour
dc.contributor.author Nagy, Sandor
dc.contributor.author Taller, Janos
dc.contributor.author Polgar, Zsolt
dc.date.accessioned 2014-01-06T11:22:10Z
dc.date.available 2014-01-06T11:22:10Z
dc.date.issued 2010
dc.identifier.citation Poczai , P , Cernak , I , Gorji , A M , Nagy , S , Taller , J & Polgar , Z 2010 , ' Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae) ' , American Journal of Botany , vol. 97 , pp. E142-E145 . https://doi.org/10.3732/ajb.1000360
dc.identifier.other PURE: 21024143
dc.identifier.other PURE UUID: 71b93f42-31d5-44fa-b7ac-59f883c49b53
dc.identifier.other WOS: 000284760800005
dc.identifier.other Scopus: 78649771000
dc.identifier.uri http://hdl.handle.net/10138/42437
dc.description.abstract • Premise of the study: Intron Targeting (IT) primers were developed for potato using expressed sequence tags (EST) and NCBI database records to study genetic diversity. • Methods and Results: Twenty-nine polymorphic intron targeting (IT) markers were generated and characterized from 30 samples of potato and 22 samples of Solanum nigrum to detect polymorphism. The number of alleles (A) per locus ranged from 2 to 7 in the analyzed populations, and the observed heterozygosity (HO) and expected heterozygosity (HE) from 0 to 0.833 and 0.750, respectively. All of the primers also amplified in the related species S. nigrum. • Conclusions: The developed markers will provide valuable tools for genetic diversity analysis, genetic mapping, and marker-assisted breeding of potato and related Solanum species. en
dc.format.extent 4
dc.language.iso eng
dc.relation.ispartof American Journal of Botany
dc.rights.uri info:eu-repo/semantics/restrictedAccess
dc.subject genetic diversity
dc.subject intron targeting
dc.subject potato
dc.subject Solanum nigrum
dc.subject transferability
dc.subject 1181 Ecology, evolutionary biology
dc.title Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae) en
dc.type Article
dc.contributor.organization Biosciences
dc.contributor.organization Embryophylo
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3732/ajb.1000360
dc.relation.issn 0002-9122
dc.rights.accesslevel restrictedAccess
dc.type.version submittedVersion
dc.identifier.url http://www.amjbot.org/content/97/12/e142.short

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