Diversity of long terminal repeat retrotransposon genome distribution in natural populations of the wild diploid wheat Aegilops speltoides

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dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor.author Hosid, Elena
dc.contributor.author Brodsky, Leonid
dc.contributor.author Kalendar, Ruslan
dc.contributor.author Raskina, Olga
dc.contributor.author Belyayev, Alexander
dc.date.accessioned 2014-02-05T22:08:26Z
dc.date.available 2014-02-05T22:08:26Z
dc.date.issued 2012
dc.identifier.citation Hosid , E , Brodsky , L , Kalendar , R , Raskina , O & Belyayev , A 2012 , ' Diversity of long terminal repeat retrotransposon genome distribution in natural populations of the wild diploid wheat Aegilops speltoides ' , Genetics , vol. 190 , no. 1 , pp. 263-274 . https://doi.org/10.1534/genetics.111.134643 en
dc.identifier.issn 0016-6731
dc.identifier.other PURE: 9343795
dc.identifier.other PURE UUID: a0f1dcda-b5dc-4829-a794-e094e97ae9b8
dc.identifier.other WOS: 000299166800018
dc.identifier.other Scopus: 84855418290
dc.identifier.other ORCID: /0000-0003-3986-2460/work/43649606
dc.identifier.uri http://hdl.handle.net/10138/42722
dc.description.abstract Background: The environment can have a decisive influence on the structure of the genome, changing it in a certain direction. Therefore, the genomic distribution of environmentally sensitive transposable elements may vary measurably across a species area. In the present research, we aimed to detect and evaluate the level of LTR retrotransposon intraspecific variability in Aegilops speltoides (2n=2x=14), a wild cross-pollinated relative of cultivated wheat. Results: The inter-retrotransposon amplified polymorphism (IRAP) protocol was applied to detect and evaluate the level of LTR retrotransposon intraspecific variability in Ae. speltoides and closely related species of sect. Sitopsis. IRAP analysis revealed significant diversity in TE distribution. Various genotypes from the same population significantly differ with respect to the patterns of the four explored LTR retrotransposons (WIS2, Wilma, Daniela, and Fatima). This diversity points to a constant ongoing process of LTR retrotransposon fraction restructuring in populations of Ae. speltoides throughout the species’ range and within single populations in time. Maximum changes were recorded in genotypes from small stressed populations. Principal component analysis showed that the dynamics of the Fatima element in populations of Ae. speltoides significantly differ from those of WIS2, Wilma, and Daniela. In terms of relationships between Sitopsis species, IRAP analysis of WIS2, Wilma, and Daniela elements revealed a grouping similar to groupings determined by other methods, with Ae. sharonensis and Ae. longissima forming a separate unit, Ae. speltoides appearing as a dispersed group, and Ae. bicornis being in an intermediate position. Conclusions: IRAP display data revealed dynamic changes in LTR retrotransposon fractions in the genome of Ae. speltoides. The process is permanent and population-specific, ultimately leading to the separation of small stressed populations from the main bunch. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof Genetics
dc.relation.uri http://www.genetics.org/content/190/1/263.full
dc.rights en
dc.subject 1181 Ecology, evolutionary biology en
dc.subject Aegilops en
dc.subject IRAP en
dc.subject retrotransposons en
dc.subject populations en
dc.subject wheat en
dc.title Diversity of long terminal repeat retrotransposon genome distribution in natural populations of the wild diploid wheat Aegilops speltoides en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1534/genetics.111.134643
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/acceptedVersion
dc.contributor.pbl

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