Sequencing the botulinum neurotoxin gene and related genes in Clostridium botulinum type E strains reveals orfx3 and a novel type E neurotoxin subtype.

Show simple item record Chen, Ying en Korkeala, Hannu en Aarnikunnas, Johannes en Lindstrom, Miia en
dc.contributor.other Department of Food and Environmental Hygiene, P.O. Box 66, FIN-00014, University of Helsinki, Finland. en 2008-01-07T13:43:53Z en 2009-06-17T14:03:42Z 2008-01-07T13:43:53Z en 2009-06-17T14:03:42Z 2007 en
dc.identifier.citation J Bacteriol. 2007 Dec;189(23):8643-50. Epub 2007 Sep 28. en
dc.identifier.issn 1098-5530 (Electronic) en
dc.identifier.uri en
dc.description.abstract Three Clostridium botulinum type E strains were sequenced for the botulinum neurotoxin (BoNT) gene cluster, and 11 type E strains, representing a wide biodiversity, were sequenced for the bont/E gene. The total length of the BoNT/E gene cluster was 12,908 bp, and a novel gene (partial) designated orfx3, together with the complete orfx2 gene, was identified in the three type E strains for the first time. Apart from orfx3, the structure and organization of the neurotoxin gene cluster of the three strains were identical to those of previously published ones. Only minor differences (</=3%) in the nucleotide sequences of the gene cluster components were observed among the three strains and the published BoNT/E-producing clostridia. The orfx3, orfx2, orfx1, and p47 gene sequences of the three type E strains shared homologies of 81%, 67 to 76%, 78 to 79%, and 79 to 85%, respectively, with published sequences for type A1 and A2 C. botulinum. Analysis of bont/E from the 14 type E strains and 19 previously published BoNT/E-producing clostridia revealed six neurotoxin subtypes, with a new distinct subtype consisting of three Finnish isolates alone. The amino acid sequence of the subtype E6 neurotoxin differed 3 to 6% from the other subtypes, suggesting that these subtype E6 neurotoxins may possess specific antigenic or functional properties. en
dc.language eng en
dc.subject.mesh Amino Acid Sequence en
dc.subject.mesh Botulinum Toxins/chemistry/*classification/*genetics/metabolism en
dc.subject.mesh Clostridium botulinum/*classification/*genetics en
dc.subject.mesh Genes, Bacterial/*genetics en
dc.subject.mesh Multigene Family en
dc.subject.mesh Open Reading Frames/*genetics en
dc.subject.mesh Phylogeny en
dc.subject.mesh Sequence Homology en
dc.subject.mesh Variation (Genetics) en
dc.title Sequencing the botulinum neurotoxin gene and related genes in Clostridium botulinum type E strains reveals orfx3 and a novel type E neurotoxin subtype. en
dc.type Journal Article en
dc.type Research Support, Non-U.S. Gov't en
dc.identifier.laitoskoodi E9003 fi
dc.creator.corporateName Elintarvike- ja ympäristöhygienian laitos fi
dc.creator.corporateName Department of Food and Environmental Hygiene en
dc.creator.corporateName Livsmedels- och miljöhygien, Institutionen för sv
dc.identifier.pmid 17905976 en
dc.identifier.journal Journal of bacteriology en

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