2026-09-22T23:17:51Zhttps://helda.helsinki.fi/server/oai/request

oai:helda.helsinki.fi:10138/3580562026-03-27T00:31:37Zcom_10138_18086com_10138_17738col_10138_18088

Helsingin yliopisto, Bio- ja ympäristötieteellinen tiedekunta University of Helsinki, Faculty of Biological and Environmental Sciences Helsingfors universitet, Bio- och miljövetenskapliga fakulteten Koskinen, Elisabeth Dr. Jenni Prokkola, Dr. Anita Wagner 2023 URN:NBN:fi:hulib-202305242130 http://hdl.handle.net/10138/358056 Three-dimensional (3D) cellular cultures have been shown to represent tissue formations and functions more accurately than two-dimensional (2D) cultures and have been successfully utilized more accurately in model organisms, e.g., to understand cellular modular functions. However, the applications in non-model organisms are limited, and to our knowledge have not been implemented in ectotherms. At an ecological scale, the technique can enhance our understanding by providing insights on cellular and tissue level molecular mechanisms. A potential implementation of this method in Atlantic salmon is to elucidate the molecular function of the vestigial-like 3 (vgll3) gene, which plays a central role in salmonid maturity, potentially by regulating energy allocation via regulating adipogenesis. In this thesis, a proof of principle study was implemented, where the feasibility of obtaining and maintaining a suspension 3D adipose tissue culture in Atlantic salmon (Salmo salar) was assessed. The harvested visceral white adipose tissue from around the intestinal tissue of mature Atlantic salmon (salmon past smolt stage) was first separated into stromal vascular fraction (SVFs) and mature adipose fraction (MAFs). SVFs contain preadipocytes (precursors of adipocyte cells) in addition to a variety of other cell types. MAFs are mature adipocytes. Both MAFs and SVFs were successfully maintained in-vitro for over four weeks. SVFs were then successfully differentiated into mature adipocytes, demonstrating the feasibility of studying adipogenesis in Atlantic salmon. Proof of this methodology and its further implications may help us to understand the cellular functions of vgll3 and may subsequently help to better understand its causal relation to the maturation process in Atlantic salmon. eng Helsingin yliopisto University of Helsinki Helsingfors universitet Atlantic salmon 3D cell culture adipose tissue adipocyte differentiation age-at-maturity ei opintosuuntaa no specialization ingen studieinriktning Ekologian ja evoluutiobiologian maisteriohjelma Master's Programme in Ecology and Evolutionary Biology Magisterprogrammet i ekologi och evolutionsbiologi Building an In-vitro Cell Model to Study Cellular Basis of Maturation Timing: A Proof of 3D Cell Culture Methodology in Atlantic Salmon Adipose Tissue pro gradu -tutkielmat master's thesis pro gradu-avhandlingar restrictedAccess URN:NBN:fi:hulib-202305242130 restricted