Spoljaric, InkeriSpoljaric, AlbertMavrovic, MartinaSeja, PatriciaPuskarjov, MartinKaila, Kai2019-03-122019-03-122019-01-29Spoljaric, I, Spoljaric, A, Mavrovic, M, Seja, P, Puskarjov, M & Kaila, K 2019, 'KCC2-Mediated Cl- Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice', Cell Reports, vol. 26, no. 5, pp. 1073-+. https://doi.org/10.1016/j.celrep.2019.01.011ORCID: /0000-0003-0668-5955/work/55352738ORCID: /0000-0002-8736-5947/work/55353611ORCID: /0000-0001-9111-6946/work/55353665http://hdl.handle.net/10138/300050It is generally thought that hippocampal neurons of perinatal rats and mice lack transport-functional K-Cl cotransporter KCC2, and that Cl- regulation is dominated by Cl- uptake via the Na-K-2Cl cotransporter NKCC1. Here, we demonstrate a robust enhancement of spontaneous hippocampal network events (giant depolarizing potentials [GDPs]) by the KCC2 inhibitor VU0463271 in neonatal rats and late-gestation, wildtype mouse embryos, but not in their KCC2-null littermates. VU0463271 increased the depolarizing GABAergic synaptic drive onto neonatal CA3 pyramidal neurons, increasing their spiking probability and synchrony during the rising phase of a GDP. Our data indicate that Cl- extrusion by KCC2 is involved in modulation of GDPs already at their developmental onset during the perinatal period in mice and rats.12engcc_by_nc_ndinfo:eu-repo/semantics/openAccessGIANT DEPOLARIZING POTENTIALSHUB NEURONSPYRAMIDAL NEURONSGABAERGIC ACTIONSPERFORATED-PATCHKCC2 EXPRESSIONCOTRANSPORTERGABAINTERNEURONSACTIVATIONBiochemistry, cell and molecular biologyArticleopenAccess2e6cae88-9a4f-4dad-9fb3-a6f3d45af21885060456816000456952400001