Hiippala, KaisaKhan, ImranRonkainen, AkiFredrik, BoulundVähä-Mäkilä, HelenaSuutarinen, Maiju ElinaSeifert, MaikeEngstrand, LarsSatokari, Reetta2022-02-252022-02-252022-01-01Hiippala, K, Khan, I, Ronkainen, A, Fredrik, B, Vähä-Mäkilä, H, Suutarinen, M E, Seifert, M, Engstrand, L & Satokari, R 2022, 'Novel strain of Pseudoruminococcus massiliensis possesses traits important in gut adaptation and host-microbe interactions', Gut Microbes, vol. 14, no. 1, 2013761. https://doi.org/10.1080/19490976.2021.2013761ORCID: /0000-0002-3206-3386/work/108869120http://hdl.handle.net/10138/341040Fecal microbiota transplantation (FMT) is an efficient treatment for recurrent Clostridioides difficile infection and currently investigated as a treatment for other intestinal and systemic diseases. Better understanding of the species potentially transferred in FMT is needed. We isolated from a healthy fecal donor a novel strain E10-96H of Pseudoruminococcus massiliensis, a recently described strictly anaerobic species currently represented only by the type strain. The whole genome sequence of E10-96H had over 98% similarity with the type strain. E10-96H carries 20 glycoside hydrolase encoding genes, degrades starch in vitro and thus may contribute to fiber degradation, cross-feeding of other species and butyrate production in the intestinal ecosystem. The strain carries pilus-like structures, harbors pilin genes in its genome and adheres to enterocytes in vitro but does not provoke a proinflammatory response. P. massiliensis seems to have commensal behavior with the host epithelium, and its role in intestinal ecology should be studied further.13engcc_byinfo:eu-repo/semantics/openAccess11832 Microbiology and virologyadhesionpiliPseudoruminococcusgut microbiotanext-generation probioticsstarch degradationFMTPseudoruminococcuspiliadhesiongut microbiotanext-generation probioticstarch degradationFMTENGRAFTMENTALGORITHMALIGNMENTREVEALSSTARCHPILIArticleopenAccess72b6f452-cb27-4b5e-93fb-f6a2240404db000735827500001