Stape, Thiago Henrique ScarabelloTjäderhane, LeoAbuna, GabrielSinhoreti, Mário Alexandre CoelhoMartins, Luís Roberto MarcondesTezvergil-Mutluay, Arzu2019-05-312025-08-272018-07Stape, T H S, Tjäderhane, L, Abuna, G, Sinhoreti, M A C, Martins, L R M & Tezvergil-Mutluay, A 2018, 'Optimization of the etch-and-rinse technique : New perspectives to improve resin–dentin bonding and hybrid layer integrity by reducing residual water using dimethyl sulfoxide pretreatments', Dental Materials, vol. 34, no. 7, pp. 967-977. https://doi.org/10.1016/j.dental.2018.03.010RIS: urn:EE70B941647367963EA838D56BC932B3ORCID: /0000-0002-5018-478X/work/46037593http://hdl.handle.net/10138/302394Objective. To determine whether bonding effectiveness and hybrid layer integrity on acid-etched dehydrated dentin would be comparable to the conventional wet-bonding technique through new dentin biomodification approaches using dimethyl sulfoxide (DMSO). Methods. Etched dentin surfaces from extracted sound molars were randomly bonded in wet or dry conditions (30 s air drying) with DMSO/ethanol or DMSO/H2O as pretreatments using a simplified (Scotchbond Universal Adhesive, 3M ESPE: SU) and a multi-step (Adper Scotchbond Multi-Purpose, 3M ESPE: SBMP) etch-and-rinse adhesives. Untreated dentin surfaces served as control. Bonded teeth (n=8) were stored in distilled water for 24 h and sectioned into resin-dentin beams (0.8 mm(2)) for microtensile bond strength test and quantitative interfacial nanoleakage analysis (n = 8) under SEM. Additional teeth (n = 2) were prepared for micropermeability assessment by CFLSM under simulated pulp ar pressure (20 cm H2O) using 5 mM fluorescein as a tracer. Microtensile data was analyzed by 3-way ANOVA followed by Tukey Test and nanoleakage by Kruskal-Wallis and Dunn-Bonferroni multiple comparison test (alpha = 0.05). Results. While dry-bonding of SBMP produced significantly lower bond strengths than wet-bonding (p Conclusion. DMSO pretreatments may be used as a new suitable strategy to improve bonding of water-based adhesives to demineralized air-dried dentin beyond conventional wetbonding. Less porous resin-dentin interfaces with higher bond strengths on air-dried etched dentin were achieved; nonetheless, overall efficiency varied according to DMSO's co-solvent and adhesive type. Clinical significance. DMSO pretreatments permit etched dentin to be air-dried before hybridization facilitating residual water removal and thus improving bonding effectiveness. This challenges the current paradigm of wet-bonding requirement for the etch-and-rinse approach creating new possibilities to enhance the clinical longevity of resin-dentin interfaces. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.11enginfo:eu-repo/semantics/openAccessDry-bondingWet-bondingBond strengthMicropermeabilityNanoleakageDentinDMSOEthanolAdhesivesNONCARIOUS CERVICAL LESIONSQUALITYRETENTIONWETDRYMICROSCOPYSOLVENTSTRENGTHSIN-VITROADHESIVE RESINDentistryArticleopenAccess532c5fc7-7319-42fd-a316-a10b29eda27685045345542000434463000006