TY - T1 - A Strong Contractile Actin Fence and Large Adhesions Direct Human Pluripotent Colony Morphology and Adhesion SN - / UR - http://hdl.handle.net/10138/204689 T3 - A1 - Narva, Elisa; Stubb, Aki; Guzman, Camilo; Blomqvist, Matias; Balboa, Diego; Lerche, Martina; Saari, Markku; Otonkoski, Timo; Ivaska, Johanna A2 - PB - Y1 - 2017 LA - eng AB - Cell-type-specific functions and identity are tightly regulated by interactions between the cell cytoskeleton and the extracellular matrix (ECM). Human pluripotent stem cells (hPSCs) have ultimate differentiation capacity and exceptionally low-strength ECM contact, yet the organization and function of adhesion sites and associated actin cytoskeleton remain poorly defined. We imaged hPSCs at the cell-ECM interface with total internal reflection fluorescence microscopy and discovered that adhesion... VO - IS - SP - OP - KW - EMBRYONIC STEM-CELLS; SELF-RENEWAL; RECOMBINANT VITRONECTIN; INTEGRIN; SRC; RHO; DIFFERENTIATION; CULTURE; ORGANIZATION; EXPANSION; 3111 Biomedicine N1 - PP - ER -