TY  -  
T1  - A high-throughput method for orthophosphate determination of thermostable membrane-bound pyrophosphatase activity 
SN  -  /  
UR  - http://hdl.handle.net/10138/237234 
T3  -  
A1  - Vidilaseris, Keni; Kellosalo, Juho; Goldman, Adrian 
A2  -  
PB  -  
Y1  - 2018 
LA  - eng 
AB  - Membrane-bound pyrophosphatases (mPPases) are homodimeric integral membrane proteins that hydrolyse pyrophosphate into orthophosphates coupled to the active transport of protons or sodium ions across membranes. They occur in bacteria, archaea, plants, and protist parasites. As they are essential in protist parasites and there are no homologous proteins in animals and humans, these enzymes represent an excellent drug target for treating protistal diseases. Experimental screening to find drug cand... 
VO  -  
IS  -  
SP  -  
OP  -  
KW  - LABILE ORGANIC PHOSPHATE; COLI INORGANIC PYROPHOSPHATASE; COLORIMETRIC DETERMINATION; THERMOTOGA-MARITIMA; HETEROLOGOUS EXPRESSION; PUMPING PYROPHOSPHATASE; CRYSTAL-STRUCTURE; ASSAY; ACIDOCALCISOMES; PURIFICATION; 116 Chemical sciences 
N1  -   
PP  -  
ER  -