TY  -  
T1  - An efficient auxin-inducible degron system with low basal degradation in human cells 
SN  -  /  
UR  - http://hdl.handle.net/10138/319568 
T3  -  
A1  - Li, Shiqian; Prasanna, Xavier; Salo, Veijo T.; Vattulainen, Ilpo; Ikonen, Elina 
A2  -  
PB  -  
Y1  - 2019 
LA  - eng 
AB  - Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron minilAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust fu... 
VO  -  
IS  -  
SP  -  
OP  -  
KW  - DEPLETION; PROTEINS; DRIVEN; 3111 Biomedicine; 1182 Biochemistry, cell and molecular biology 
N1  -   
PP  -  
ER  -