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T1  - An improved RT-qPCR method for direct quantification of enveloped RNA viruses 
SN  -  /  
UR  - http://hdl.handle.net/10138/344424 
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A1  - Gregorova, Pavlina; Heinonen, Minna-Maria Kristiina; Sarin, Peter 
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PB  -  
Y1  - 2022 
LA  - eng 
AB  - Reverse transcription quantitative PCR (RT-qPCR) has emerged as the gold standard for virus detection and quantification, being utilized in numerous diagnostic and research applications. However, the direct detection of viruses has so far posed a challenge as the viral genome is often encapsidated by a proteinaceous layer surrounded by a lipid envelope. This necessitates an additional and undesired RNA extraction step prior to RT-qPCR amplification. To circumvent this limitation, we have develop... 
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KW  - 1182 Biochemistry, cell and molecular biology; RT-qPCR; direct detection; RNA isolation-free; 11832 Microbiology and virology; RNA virus; phage phi 6; RT-qPCR; Direct quantification; Bacteriophage; Phi6; RNA virus; RNA-dependent RNA polymerase; SARS-COV-2 
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