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T1  - Comparative analysis of cytosolic and mitochondrial ATP synthesis in embryonic and postnatal hippocampal neuronal cultures 
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UR  - http://hdl.handle.net/10138/42504 
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A1  - Surin, Alexander M.; Khiroug, Serguei; Gorbacheva, Lubov R.; Khodorov, Boris I.; Pinelis, Vsevolod G.; Khiroug, Leonard 
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Y1  - 2013 
LA  - eng 
AB  - ATP in neurons is commonly believed to be synthesized mostly by mitochondria via oxidative phosphorylation. Neuronal mitochondria have been studied primarily in culture, i.e., in neurons isolated either from embryos or from neonatal pups. Although it is generally assumed that both embryonic and postnatal cultured neurons derive their ATP from mitochondrial oxidative phosphorylation, this has never been tested experimentally. We expressed the FRET-based ATP sensor AT1.03 in cultured hippocampal n... 
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KW  - Adenosine triphosphate; F1Fo-ATPase; Fluorescent protein; OxPhos; TMRM; adenosine triphosphate; deoxyglucose; glucose; oligomycin; proton transporting adenosine triphosphate synthase; pyruvic acid; reduced nicotinamide adenine dinucleotide phosphate oxidase; animal cell; animal tissue; article; autofluorescence imaging; brain mitochondrion; cell function; controlled study; developmental stage; embryo; enzyme inhibition; fluorescence resonance energy transfer; glycolysis; mitochondrial membrane potential; nerve cell culture; nonhuman; nucleotide metabolism; oxidative phosphorylation; perinatal period; protein expression; rat; 3112 Neurosciences 
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