TY  -  
T1  - Development of amplification-based technologies for enrichment of nucleic acids with difficult sequences or low-abundance point mutations 
SN  -  /  
UR  - URN:ISBN:978-951-51-0579-0; http://hdl.handle.net/10138/153041 
T3  -  
A1  - Ho Huu, Tho 
A2  -  
PB  - Helsingin yliopisto 
Y1  - 2015 
LA  - en 
AB  - The aim of this study was to utilize molecular biology knowledge to develop novel amplification-based technologies, which enable enrichment of challenging nucleic acid targets to a level sufficient for detection, including those with extremely long, GC-rich and/or repetitive sequences and low-abundance single nucleotide RNA variants in the excess of alternative variants. 

We have introduced multiple heat pulses in the extension step of a PCR cycling protocol to generate a novel amplification te... 
VO  -  
IS  -  
SP  -  
OP  -  
KW  - medicine 
N1  -   
PP  -  
ER  -