TY - T1 - Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera) SN - / UR - http://hdl.handle.net/10138/299220 T3 - A1 - Yang, Mei; Zhu, Lingping; Li, Ling; Li, Juanjuan; Xu, Liming; Feng, Ji; Liu, Yanling A2 - PB - Y1 - 2017 LA - eng AB - The predominant alkaloids in lotus leaves are aporphine alkaloids. These are the most important active components and have many pharmacological properties, but little is known about their biosynthesis. We used digital gene expression (DGE) technology to identify differentially-expressed genes (DEGs) between two lotus cultivars with different alkaloid contents at four leaf development stages. We also predicted potential genes involved in aporphine alkaloid biosynthesis by weighted gene co-express... VO - IS - SP - OP - KW - lotus; aporphine alkaloids; co-expression network; biosynthesis; putative genes; COEXPRESSION NETWORK ANALYSIS; RNA-SEQ; MOLECULAR-CLONING; COPTIS-JAPONICA; OPIUM POPPY; BISBENZYLISOQUINOLINE ALKALOIDS; BENZYLISOQUINOLINE ALKALOIDS; HETEROLOGOUS EXPRESSION; NORCOCLAURINE SYNTHASE; SACRED LOTUS; 1183 Plant biology, microbiology, virology N1 - PP - ER -