TY  -  
T1  - Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET 
SN  -  /  
UR  - http://hdl.handle.net/10138/303899 
T3  -  
A1  - Shcherbakova, Daria M.; Cammer, Natasha Cox; Huisman, Tsipora M.; Verkhusha, Vladislav V.; Hodgson, Louis 
A2  -  
PB  -  
Y1  - 2018 
LA  - eng 
AB  - Direct visualization and light control of several cellular processes is a challenge, owing to the spectral overlap of available genetically encoded probes. Here we report the most red-shifted monomeric near-infrared (NIR) fluorescent protein, miRFP720, and the fully NIR Forster resonance energy transfer (FRET) pair miRFP670-miRFP720, which together enabled design of biosensors compatible with CFP-YFP imaging and blue-green optogenetic tools. We developed a NIR biosensor for Rac1 GTPase and demon... 
VO  -  
IS  -  
SP  -  
OP  -  
KW  - FLUORESCENT PROTEINS; LIVING CELLS; IN-VIVO; SPATIOTEMPORAL DYNAMICS; BACTERIAL PHYTOCHROME; CDC42 ACTIVATION; BIOSENSORS; RAC; TOOLS; COORDINATION; 1182 Biochemistry, cell and molecular biology; 3111 Biomedicine 
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PP  -  
ER  -