TY - T1 - Effect of RNA quality to SARS-CoV-2 RT-qPCR detection from saliva SN - / UR - http://hdl.handle.net/10138/343821 T3 - A1 - Paju, Susanna; Tallgren, Marika; Kivimäki, Anne; Lahdentausta, Laura; Salminen, Aino; Oksanen, Lotta-Maria Adele Helena; Sanmark, Enni; Geneid, Ahmed; Pussinen, Pirkko; Pietiäinen, Milla A2 - PB - Y1 - 2022 LA - eng AB - Saliva is an alternative sample material to nasopharyngeal swab in SARS- CoV- 2 diagnostics. We investigated possible aspects to improve the reliability of SARS- CoV- 2 detection from saliva. Saliva was collected from asymptomatic healthy subjects (n=133) and COVID- 19 patients (n=9). SARS- CoV- 2 detection was performed with quantitative reverse- transcriptase PCR (RT- qPCR) with two viral and one host target serving as an internal control. The use of internal control revealed that in the first... VO - IS - SP - OP - KW - 11832 Microbiology and virology; susanna; paju@helsinki; fi SARS-CoV-2; COVID-19; RT-qPCR; saliva N1 - PP - ER -